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Electromagnetic fields induce neural differentiation of human bone marrow derived mesenchymal stem cells via ROS mediated EGFR activation.

Neurochemistry international · 2013 · Benefit · Low evidence

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BenefitLow evidencePaper 4624

In hBM-MSCs exposed to ELF-EMF (50 Hz, 1 mT), neural markers (NF-L, MAP2, NeuroD1) increased at 6 days. Akt and CREB phosphorylation increased at 90 min while ERK did not; EGFR phosphorylation increased at 90 min, and pretreatment with N-acetylcysteine or the…

Study snapshot

PublicationNeurochemistry international · 2013
Study typein_vitro
Reported directionBenefit
Evidence ratingLow

Abstract

Even though the inducing effect of electromagnetic fields (EMF) on the neural differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs) is a distinctive, the underlying mechanism of differentiation remains unclear. To find out the signaling pathways involved in the neural differentiation of BM-MSCs by EMF, we examined the CREB phosphorylation and Akt or ERK activation as an upstream of CREB. In hBM-MSCs treated with ELF-EMF (50 Hz, 1 mT), the expression of neural markers such as NF-L, MAP2, and NeuroD1 increased at 6 days and phosphorylation of Akt and CREB but not ERK increased at 90 min in BM-MSCs. Moreover, EMF increased phosphorylation of epidermal growth factor receptor (EGFR) as an upstream receptor tyrosine kinase of PI3K/Akt at 90 min. It has been well documented that ELF-MF exposure may alter cellular processes by increasing intracellular reactive oxygen species (ROS) concentrations. Thus, we examined EMF-induced ROS production in BM-MSCs. Moreover, pretreatment with a ROS scavenger, N-acetylcystein, and an EGFR inhibitor, AG-1478, prevented the phosphorylation of EGFR and downstream molecules. These results suggest that EMF induce neural differentiation through activation of EGFR signaling and mild generation of ROS.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationHuman bone marrow-derived mesenchymal stem cells (hBM-MSCs)
Sample sizeNot supplied
ExposureELF · 0.05 MHz · 90 min (signaling endpoints); 6 days (neural marker expression)
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In hBM-MSCs exposed to ELF-EMF (50 Hz, 1 mT), neural markers (NF-L, MAP2, NeuroD1) increased at 6 days. Akt and CREB phosphorylation increased at 90 min while ERK did not; EGFR phosphorylation increased at 90 min, and pretreatment with N-acetylcysteine or the EGFR inhibitor AG-1478 prevented EGFR and downstream phosphorylation, consistent with ROS-mediated EGFR signaling involvement.

Outcomes measured

  • Neural differentiation markers (NF-L, MAP2, NeuroD1)
  • Akt phosphorylation
  • CREB phosphorylation
  • ERK activation
  • EGFR phosphorylation
  • Reactive oxygen species (ROS) production
  • Effects of N-acetylcysteine (ROS scavenger) and AG-1478 (EGFR inhibitor) on signaling

Limitations

  • In vitro study; findings may not translate to in vivo or clinical outcomes
  • Sample size not reported in the provided abstract
  • Exposure conditions limited to 50 Hz, 1 mT; generalizability to other field strengths/frequencies unclear
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "90 min (signaling endpoints); 6 days (neural marker expression)"
  },
  "population": "Human bone marrow-derived mesenchymal stem cells (hBM-MSCs)",
  "sample_size": null,
  "outcomes": [
    "Neural differentiation markers (NF-L, MAP2, NeuroD1)",
    "Akt phosphorylation",
    "CREB phosphorylation",
    "ERK activation",
    "EGFR phosphorylation",
    "Reactive oxygen species (ROS) production",
    "Effects of N-acetylcysteine (ROS scavenger) and AG-1478 (EGFR inhibitor) on signaling"
  ],
  "main_findings": "In hBM-MSCs exposed to ELF-EMF (50 Hz, 1 mT), neural markers (NF-L, MAP2, NeuroD1) increased at 6 days. Akt and CREB phosphorylation increased at 90 min while ERK did not; EGFR phosphorylation increased at 90 min, and pretreatment with N-acetylcysteine or the EGFR inhibitor AG-1478 prevented EGFR and downstream phosphorylation, consistent with ROS-mediated EGFR signaling involvement.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study; findings may not translate to in vivo or clinical outcomes",
    "Sample size not reported in the provided abstract",
    "Exposure conditions limited to 50 Hz, 1 mT; generalizability to other field strengths/frequencies unclear"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "1 mT",
    "mesenchymal stem cells",
    "neural differentiation",
    "ROS",
    "EGFR",
    "PI3K/Akt",
    "CREB",
    "NF-L",
    "MAP2",
    "NeuroD1",
    "N-acetylcysteine",
    "AG-1478"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.neuint.2013.02.002
PubMed: 23411410

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