Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells.

Experimental biology and medicine (Maywood, N.J.) · 2013 · Benefit · Low evidence

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BenefitLow evidencePaper 4582

BM-MSCs exposed to a 50-Hz ELF-EMF during in vitro expansion showed a significantly decreased proliferation rate and an increase in neuronal differentiation. ELF-EMF-treated cells had increased MAP2 and down-regulated Nestin, with elevated intracellular Ca(2+…

Study snapshot

PublicationExperimental biology and medicine (Maywood, N.J.) · 2013

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

Extremely low-frequency electromagnetic fields (ELF-EMF) affect numerous biological functions such as gene expression, cell fate determination and even cell differentiation. To investigate the correlation between ELF-EMF exposure and differentiation, bone marrow derived mesenchymal stem cells (BM-MSCs) were subjected to a 50-Hz electromagnetic field during in vitro expansion. The influence of ELF-EMF on BM-MSCs was analysed by a range of different analytical methods to understand its role in the enhancement of neural differentiation. ELF-EMF exposure significantly decreased the rate of proliferation, which in turn caused an increase in neuronal differentiation. The ELF-EMF-treated cells showed increased levels of neuronal differentiation marker (MAP2), while early neuronal marker (Nestin) was down-regulated. In addition, eight differentially expressed proteins were detected in two-dimensional electrophoresis maps, and were identified using ESI-Q-TOF LC/MS/MS. Among them, ferritin light chain, thioredoxin-dependent peroxide reductase, and tubulin β-6 chain were up-regulated in the ELF-EMF-stimulated group. Ferritin and thioredoxin-dependent peroxide reductase are involved in a wide variety of functions, including Ca(2+) regulation, which is a critical component of neurodegeneration. We also observed that the intracellular Ca(2+) content was significantly elevated after ELF-EMF exposure, which strengthens the modulatory role of ferritin and thioredoxin-dependent peroxide reductase, during differentiation. Notably, western blot analysis indicated significantly increased expression of the ferritin light chain in the ELF-EMF-stimulated group (0.60 vs. 1.08; P < 0.01). These proteins may help understand the effect of ELF-EMF stimulation on BM-MSCs during neural differentiation and its potential use as a clinically therapeutic option for treating neurodegenerative diseases.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationBone marrow derived mesenchymal stem cells (BM-MSCs)

Sample sizeNot supplied

ExposureELF · 0.05 MHz · during in vitro expansion

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Cell proliferation rate
Neural/neuronal differentiation
Expression of neuronal differentiation marker MAP2
Expression of early neuronal marker Nestin
Differentially expressed proteins (2D electrophoresis; ESI-Q-TOF LC/MS/MS identification)
Intracellular Ca(2+) content
Ferritin light chain expression (western blot)

Limitations

In vitro study; findings may not translate to in vivo or clinical outcomes
Exposure parameters beyond frequency (e.g., field strength) not reported in abstract
Sample size not reported in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "during in vitro expansion"
  },
  "population": "Bone marrow derived mesenchymal stem cells (BM-MSCs)",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation rate",
    "Neural/neuronal differentiation",
    "Expression of neuronal differentiation marker MAP2",
    "Expression of early neuronal marker Nestin",
    "Differentially expressed proteins (2D electrophoresis; ESI-Q-TOF LC/MS/MS identification)",
    "Intracellular Ca(2+) content",
    "Ferritin light chain expression (western blot)"
  ],
  "main_findings": "BM-MSCs exposed to a 50-Hz ELF-EMF during in vitro expansion showed a significantly decreased proliferation rate and an increase in neuronal differentiation. ELF-EMF-treated cells had increased MAP2 and down-regulated Nestin, with elevated intracellular Ca(2+) and increased ferritin light chain expression (0.60 vs. 1.08; P<0.01).",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study; findings may not translate to in vivo or clinical outcomes",
    "Exposure parameters beyond frequency (e.g., field strength) not reported in abstract",
    "Sample size not reported in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "mesenchymal stem cells",
    "BM-MSCs",
    "neural differentiation",
    "neuronal markers",
    "MAP2",
    "Nestin",
    "proteomics",
    "ferritin light chain",
    "thioredoxin-dependent peroxide reductase",
    "intracellular calcium"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1177/1535370213497173
PubMed: 23970408

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