Combined effects of flow-induced shear stress and electromagnetic field on neural differentiation of mesenchymal stem cells.

Electromagnetic biology and medicine · 2016 · Benefit · Very low evidence

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BenefitVery low evidencePaper 4753

A 1 h induction combining flow-induced shear stress and electromagnetic field exposure led to detection of the neural precursor marker nestin as early as 9 h after treatment, persisting for the following 6 days. The combined treatment produced neurite-like ce…

Study snapshot

PublicationElectromagnetic biology and medicine · 2016

Study typein_vitro

Reported directionBenefit

Evidence ratingVery low

Abstract

Differentiation of bone marrow-derived mesenchymal stem cells (MSCs) into neural phenotype has been induced by either flow-induced shear stress (FSS) or electromagnetic fields (EMF). However, procedures are still expensive and time consuming. In the present work, induction for 1 h with the combination of both forces showed the presence of the neural precursor nestin as early as 9 h in culture after treatment and this result lasted for the following 6 d. In conclusion, the use of a combination of FSS and EMF for a short-time renders in neurite-like cells, although further investigation is required to analyze cell functionality.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationNot supplied

Sample sizeNot supplied

Exposure1 h

Evidence strengthVery lowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Neural differentiation of bone marrow-derived mesenchymal stem cells (MSC)
Nestin (neural precursor marker) expression
Neurite-like cell morphology

Limitations

No EMF exposure parameters reported (e.g., frequency, intensity, waveform)
Functional characterization of differentiated cells not assessed (authors state further investigation is required)
No sample size reported

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "1 h"
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Neural differentiation of bone marrow-derived mesenchymal stem cells (MSC)",
    "Nestin (neural precursor marker) expression",
    "Neurite-like cell morphology"
  ],
  "main_findings": "A 1 h induction combining flow-induced shear stress and electromagnetic field exposure led to detection of the neural precursor marker nestin as early as 9 h after treatment, persisting for the following 6 days. The combined treatment produced neurite-like cells; the authors note further work is needed to assess functionality.",
  "effect_direction": "benefit",
  "limitations": [
    "No EMF exposure parameters reported (e.g., frequency, intensity, waveform)",
    "Functional characterization of differentiated cells not assessed (authors state further investigation is required)",
    "No sample size reported"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "mesenchymal stem cells",
    "bone marrow-derived",
    "neural differentiation",
    "electromagnetic field",
    "flow-induced shear stress",
    "nestin",
    "neurite-like cells"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3109/15368378.2015.1036068
PubMed: 26325339

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