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[Exposure to power-frequency magnetic fields can induce activation of P38 mitogen-activated protein kinase].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases · 2002 · Mixed · Low evidence

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MixedLow evidencePaper 3805

Exposure to 50 Hz magnetic fields at 0.4 mT for 10 minutes transiently increased phosphorylation (activation) of P38 MAPK, returning to control levels by 15 minutes. Exposure at 0.1 mT did not induce P38 MAPK activation within 24 hours. Neither 0.1 mT nor 0.4…

Study snapshot

PublicationZhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases · 2002
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

OBJECTIVE: To study the effects of 50 Hz power-frequency magnetic fields on signal transduction pathway of P38 mitogen-activated protein kinase (P38 MAPK), and explore the cellular signal transduction mechanism of the biological effects induced by power-frequency magnetic fields. METHODS: Chinese hamster lung (CHL) cell line was exposed to power-frequency magnetic fields with two intensities(0.1 and 0.4 mT) for different exposure durations. The cytoplasmic protein was extracted. The phosphorylated(activated) and non-phosphorylated P38 MAPK and MKK3/MKK6 were measured by Western blotting analysis with their specific corresponding antibodies. RESULTS: Power-frequency magnetic fields at 0.4 mT for 10 min could transitorily induce the activation of P38 MAPK and after 15 min the phosphorylation of P38 MAPK restored to control level, while 0.1 mT power-frequency magnetic fields could not induce the activation of P38 MAPK within 24 h. However, both 0.1 mT and 0.4 mT power-frequency magnetic fields could not phosphorylate(activate) the MKK3/MKK6, which is a general upstream kinase of P38 MAPK. CONCLUSION: Power-frequency magnetic fields could transitorily activate the P38 MAPK, but not MKK3/MKK6. The activation mechanism of P38 MAPK needs to be further identified.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationChinese hamster lung (CHL) cell line
Sample sizeNot supplied
ExposureELF · 0.05 MHz · 10 min; up to 24 h (depending on condition)
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Exposure to 50 Hz magnetic fields at 0.4 mT for 10 minutes transiently increased phosphorylation (activation) of P38 MAPK, returning to control levels by 15 minutes. Exposure at 0.1 mT did not induce P38 MAPK activation within 24 hours. Neither 0.1 mT nor 0.4 mT exposures activated (phosphorylated) the upstream kinases MKK3/MKK6.

Outcomes measured

  • Phosphorylation (activation) of P38 MAPK
  • Phosphorylation (activation) of MKK3/MKK6

Limitations

  • Sample size not reported in abstract
  • Only one cell line studied (CHL)
  • Limited endpoints (Western blot phosphorylation of selected kinases)
  • Exposure conditions and replication details not provided in abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "10 min; up to 24 h (depending on condition)"
  },
  "population": "Chinese hamster lung (CHL) cell line",
  "sample_size": null,
  "outcomes": [
    "Phosphorylation (activation) of P38 MAPK",
    "Phosphorylation (activation) of MKK3/MKK6"
  ],
  "main_findings": "Exposure to 50 Hz magnetic fields at 0.4 mT for 10 minutes transiently increased phosphorylation (activation) of P38 MAPK, returning to control levels by 15 minutes. Exposure at 0.1 mT did not induce P38 MAPK activation within 24 hours. Neither 0.1 mT nor 0.4 mT exposures activated (phosphorylated) the upstream kinases MKK3/MKK6.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Only one cell line studied (CHL)",
    "Limited endpoints (Western blot phosphorylation of selected kinases)",
    "Exposure conditions and replication details not provided in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "power-frequency",
    "extremely low frequency",
    "magnetic field",
    "0.1 mT",
    "0.4 mT",
    "P38 MAPK",
    "MKK3",
    "MKK6",
    "signal transduction",
    "Western blot",
    "cell line"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.35,
      "reason": "Published in an industrial hygiene/occupational diseases journal and studies power-frequency magnetic fields relevant to occupational settings."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 14694646

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