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Effects of extremely low frequency electromagnetic field (50 Hz) on pentylenetetrazol-induced seizures in mice.

Acta neurologica Belgica · 2013 · Mixed · Low evidence

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MixedLow evidencePaper 4499

Mice exposed to 50 Hz, 0.1 mT magnetic fields for 1 day, 3 days, or 2 weeks showed no statistically significant differences in PTZ dose required to induce seizures compared with sham-exposed controls. After 1 month of exposure, the seizure induction threshold…

Study snapshot

PublicationActa neurologica Belgica · 2013
Study typeanimal
Reported directionMixed
Evidence ratingLow

Abstract

The electromagnetic fields (EMF) have various behavioral and biological effects on human body. There are growing concerns about the consequences of exposure to EMF. However, some studies have shown beneficial effects of these waves on human. In this paper, we study the effect of acute, sub acute and long-term exposure to 50 Hz, 0.1 mT magnetic fields (MF) on the seizure induction threshold in mice. 64 mice are used and divided into four groups. Eight mice in any group were selected to be exposed to MF for specific duration and the others were used as a control group. The duration of the applied exposures was as follows: (1) 1 day (acute), (2) 3 days (sub acute), (3) 2 weeks (sub acute), (4) 1 month (long term). The mice were exposed 2 h for a day. After exposure, the pentylentetrazol (PTZ) is injected to the mice to induce seizure and the needed dose for the seizure induction threshold is measured. In the acute exposure, the threshold to induce seizure in the exposed and sham-exposed groups was 44.25 and 46.5 mg, respectively, while the difference was not significant (p value = 0.5). In the sub acute exposure (3 days), the mean amount of drug to induce seizure was 47.38 mg in the exposed and 43.88 mg in the sham-exposed groups, however, the difference was not significant (p value = 0.3). The results were 52.38 and 46.75 mg after 2 weeks of exposure which were not significantly different either (p value = 0.2). After 1 month of exposure to MF, the threshold for the induction of seizure was significantly increased (p value < 0.05). The mean dosage to induce seizure in the exposed and control group was 54.3 and 45.75 mg, respectively. However, considering the p value, the difference in the seizure induction threshold between the exposed and sham-exposed groups after acute and sub acute exposure was not significant, analyzing the effects of acute, sub acute and long-term exposures totally indicates that increasing the exposure time increases the seizure induction threshold.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionmixed
PopulationMice
Sample size64
ExposureELF · 5e-05 MHz · Acute (1 day), sub-acute (3 days; 2 weeks), long-term (1 month); 2 h/day
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Mice exposed to 50 Hz, 0.1 mT magnetic fields for 1 day, 3 days, or 2 weeks showed no statistically significant differences in PTZ dose required to induce seizures compared with sham-exposed controls. After 1 month of exposure, the seizure induction threshold was significantly increased in exposed mice (mean 54.3 mg vs 45.75 mg in controls; p<0.05).

Outcomes measured

  • Pentylenetetrazol (PTZ)-induced seizure induction threshold (dose required to induce seizure)

Limitations

  • Exposure source/setup details not described in the abstract (e.g., field uniformity, sham conditions).
  • Outcome reporting is limited to mean PTZ doses and p-values; variability measures are not provided in the abstract.
  • Only one field strength (0.1 mT) and one frequency (50 Hz) are studied.
View raw extracted JSON
{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 5e-05,
    "sar_wkg": null,
    "duration": "Acute (1 day), sub-acute (3 days; 2 weeks), long-term (1 month); 2 h/day"
  },
  "population": "Mice",
  "sample_size": 64,
  "outcomes": [
    "Pentylenetetrazol (PTZ)-induced seizure induction threshold (dose required to induce seizure)"
  ],
  "main_findings": "Mice exposed to 50 Hz, 0.1 mT magnetic fields for 1 day, 3 days, or 2 weeks showed no statistically significant differences in PTZ dose required to induce seizures compared with sham-exposed controls. After 1 month of exposure, the seizure induction threshold was significantly increased in exposed mice (mean 54.3 mg vs 45.75 mg in controls; p<0.05).",
  "effect_direction": "mixed",
  "limitations": [
    "Exposure source/setup details not described in the abstract (e.g., field uniformity, sham conditions).",
    "Outcome reporting is limited to mean PTZ doses and p-values; variability measures are not provided in the abstract.",
    "Only one field strength (0.1 mT) and one frequency (50 Hz) are studied."
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF",
    "50 Hz",
    "magnetic field",
    "0.1 mT",
    "mice",
    "pentylenetetrazol",
    "PTZ",
    "seizure threshold",
    "acute exposure",
    "sub-acute exposure",
    "long-term exposure"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s13760-012-0133-y
PubMed: 23055108

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