RF Safe

Enhancement of nitric oxide generation by low frequency electromagnetic field.

Pathophysiology : the official journal of the International Society for Pathophysiology · 2000 · Harm · Low evidence

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HarmLow evidencePaper 5342

In mice, exposure to a 0.1 mT, 60 Hz electromagnetic field did not induce nitric oxide (NO) generation by itself, but significantly enhanced lipopolysaccharide (LPS)-induced NO generation in vivo as measured by ESR spectra of NO adducts in liver.

Study snapshot

PublicationPathophysiology : the official journal of the International Society for Pathophysiology · 2000
Study typeanimal
Reported directionHarm
Evidence ratingLow

Abstract

Oxidative stress is implicated in the intracellular signal transduction pathways for nitric oxide synthase (NOS) induction. The electromagnetic field (EMF) is believed to increase the free radical lifespan [S. Roy, Y. Noda, V. Eckert, M.G. Traber, A. Mori, R. Liburdy, L. Packer, The phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst in rat peritoneal neutrophils is increased by a 0.1 mT (60 Hz) magnetic field, FEBS Lett. 376 (1995) 164-6; F.S. Prato, M. Kavaliers, J.J. Carson, Behavioural evidence that magnetic field effects in the land snail, Cepaea nemoralis, might not depend on magnetite or induced electric currents, Bioelectromagnetics 17 (1996) 123-30; A.L. Hulbert, J. Metcalfe, R. Hesketh, Biological response to electromagnetic fields, FASEB 12 (1998) 395-420]. We tested the effects of EMF on endotoxin induced nitric oxide (NO) generation in vivo. Male BALB/C mice were injected with lipopolysaccharide (LPS) intraperitoneously (i.p.), followed by the exposure to EMF (0.1 mT, 60 Hz). Five hours and 30 min after the LPS administration, mice were administered with a NO spin trap, ferrous N-methyl-D-glucaminedithiocarbamate (MGD-Fe). Thirty minutes later, mice were sacrificed, and their livers were removed. The results were compared to three control groups: group A (LPS (-) EMF(-)); group B (LPS(-) EMF(+)); group C (LPS(+) EMF(-)). The ESR spectra of obtained livers were examined at room temperature. Three-line spectra of NO adducts were observed in the livers of all groups. In groups A and B very weak signals were observed, but in groups C and D strong spectra were observed. The signal intensity of the NO adducts in Group D was also significantly stronger than that in Group C. EMF itself did not induce NO generation, however, it enhanced LPS induced NO generation in vivo.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionharm
PopulationMale BALB/C mice
Sample sizeNot supplied
ExposureELF · 6e-05 MHz
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In mice, exposure to a 0.1 mT, 60 Hz electromagnetic field did not induce nitric oxide (NO) generation by itself, but significantly enhanced lipopolysaccharide (LPS)-induced NO generation in vivo as measured by ESR spectra of NO adducts in liver.

Outcomes measured

  • Nitric oxide (NO) generation in vivo (ESR detection of NO adducts in liver)

Limitations

  • Sample size not reported in abstract.
  • Exposure duration/timing details for EMF not fully specified in abstract.
  • Outcome measured in liver only; generalizability to other tissues not addressed in abstract.
  • Animal model with LPS challenge; relevance to typical human exposures not established in abstract.
View raw extracted JSON
{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 6e-05,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Male BALB/C mice",
  "sample_size": null,
  "outcomes": [
    "Nitric oxide (NO) generation in vivo (ESR detection of NO adducts in liver)"
  ],
  "main_findings": "In mice, exposure to a 0.1 mT, 60 Hz electromagnetic field did not induce nitric oxide (NO) generation by itself, but significantly enhanced lipopolysaccharide (LPS)-induced NO generation in vivo as measured by ESR spectra of NO adducts in liver.",
  "effect_direction": "harm",
  "limitations": [
    "Sample size not reported in abstract.",
    "Exposure duration/timing details for EMF not fully specified in abstract.",
    "Outcome measured in liver only; generalizability to other tissues not addressed in abstract.",
    "Animal model with LPS challenge; relevance to typical human exposures not established in abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF",
    "magnetic field",
    "60 Hz",
    "0.1 mT",
    "nitric oxide",
    "NO",
    "nitric oxide synthase",
    "NOS",
    "lipopolysaccharide",
    "LPS",
    "oxidative stress",
    "electron spin resonance",
    "ESR",
    "mouse",
    "liver"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/s0928-4680(00)00040-7
PubMed: 10927193

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