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The phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst in rat peritoneal neutrophils is increased by a 0.1 mT (60 Hz) magnetic field.

FEBS letters · 1995 · Harm · Low evidence

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HarmLow evidencePaper 4287

In the presence of a 60 Hz, 0.1 mT magnetic field, PMA-stimulated rat peritoneal neutrophils showed a 12.4% increase in DCF fluorescence compared with paired measurements without the field (n=5; 18 pairs; P<0.02).

Study snapshot

PublicationFEBS letters · 1995
Study typein_vitro
Reported directionHarm
Evidence ratingLow

Abstract

Magnetic fields (MF) may affect biological systems by increasing free radical concentrations. To test this, we have investigated whether low frequency (60 Hz) low intensity (0.1 mT) MF can modulate the phorbol 12-myristate 13- acetate (PMA) induced respiratory burst in primed rat peritoneal neutrophils, followed in real time using the dye 2',7'-dichlorofluorescin (DCFH), which reacts with free radical-derived oxidants such as H2O2 (which is formed from the dismutation of superoxide) to become 2',7'-dichlorofluorecein (DCF), a highly fluorescent compound. In the presence of the MF, a 12.4% increase in the fluorescence signal was observed in PMA-stimulated neutrophils (n = 5, P < 0.02, 18 pairs of measurements). We believe this represents the first experimental observation of MF influencing events involving free radical species generated during signal transduction in living cells.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionharm
PopulationPrimed rat peritoneal neutrophils
Sample size5
ExposureELF · 0.06 MHz
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In the presence of a 60 Hz, 0.1 mT magnetic field, PMA-stimulated rat peritoneal neutrophils showed a 12.4% increase in DCF fluorescence compared with paired measurements without the field (n=5; 18 pairs; P<0.02).

Outcomes measured

  • PMA-induced respiratory burst / oxidative burst (DCFH to DCF fluorescence as a proxy for free radical-derived oxidants such as H2O2)

Limitations

  • In vitro experiment in rat cells; generalizability to humans not addressed.
  • Small sample size (n=5) despite multiple paired measurements.
  • Outcome is an indirect fluorescence-based proxy for oxidant production (DCFH/DCF).
  • Exposure duration and other exposure conditions are not described in the abstract.
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.06,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Primed rat peritoneal neutrophils",
  "sample_size": 5,
  "outcomes": [
    "PMA-induced respiratory burst / oxidative burst (DCFH to DCF fluorescence as a proxy for free radical-derived oxidants such as H2O2)"
  ],
  "main_findings": "In the presence of a 60 Hz, 0.1 mT magnetic field, PMA-stimulated rat peritoneal neutrophils showed a 12.4% increase in DCF fluorescence compared with paired measurements without the field (n=5; 18 pairs; P<0.02).",
  "effect_direction": "harm",
  "limitations": [
    "In vitro experiment in rat cells; generalizability to humans not addressed.",
    "Small sample size (n=5) despite multiple paired measurements.",
    "Outcome is an indirect fluorescence-based proxy for oxidant production (DCFH/DCF).",
    "Exposure duration and other exposure conditions are not described in the abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF",
    "60 Hz",
    "0.1 mT",
    "magnetic field",
    "oxidative burst",
    "respiratory burst",
    "neutrophils",
    "PMA",
    "free radicals",
    "reactive oxygen species",
    "DCFH",
    "DCF"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.25,
      "reason": "Study involves ELF (60 Hz) magnetic field exposure, which is commonly relevant to occupational ELF exposure contexts, though no specific setting is stated."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/0014-5793(95)01266-x
PubMed: 7498533

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