[The laboratory detection of intra-cellular factors of anti-viral defense under community-acquired pneumonia in evaluation of effects of low-intensity microwave radiation].

Klinicheskaia laboratornaia diagnostika · 2016 · Mixed · Very low evidence

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MixedVery low evidencePaper 6594

Study snapshot

PublicationKlinicheskaia laboratornaia diagnostika · 2016

Study typein_vitro

Reported directionMixed

Evidence ratingVery low

Abstract

The study was carried out to investigate intro-cellular concentration of factors of antiviral defense of cells of whole blood of re-convalescents of community-acquired pneumonia and healthy individuals under effect of low intensity SHF-radiation of whole blood in vitro with frequency of 1000 MGz. The technique of enzyme-linked immunosorbent assay was applied for analyzing lysates of mononuclears of whole blood exposed to impact of low intensity SHF-radiation with frequency of1000 MGz on concentration of mitochondrial antiviral signal protein MAVS, RIG-I-like receptor type III - helicase LGP2, RIG-I-like receptor - helicaseMDA5, trans-membrane protein 173 (Tmem 173), interferon-regulated factors (IRF) 3,7 and 8, sub-units p50 and p65 of nuclear factor of transcription NF-kB, phosphorylated on serine in position form 32 of inhibitor of nuclear factor of transcription (IkB-a) and also its total concentration. Besides, spontaneous production of interferon - a/β by cells of whole blood was evaluated in cellular supernatant. The capacity of single 20 minutes of SHF-radiation effect to increase intracellular level of important regulative proteins, primarily MDA5, during the phase of convalescence of community-acquired pneumonia was established. Besides, radiation stimulates increasing of intracellular level of MAVS and Tmem173. The capacity of radiation was established to increase phosphorylation of inhibitor of nuclear factor NF-kB and level of components p50 and p65 of NF-kB. The capacity of SHF-radiation effecting production of interferon-β by cells of hole blood is demonstrated. In healthy individuals, radiation contributes into decreasing of intercellular content of MAVS in more degree as compared with levels of MDA5 and Tmem173. At that, radiation of culture of cells of whole blood stimulates increasing of production of interferons.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationRe-convalescents of community-acquired pneumonia and healthy individuals (whole blood samples)

Sample sizeNot supplied

Exposuremicrowave · 1000 MHz · single 20 minutes

Evidence strengthVery lowConfidence: 74% · Peer reviewed: yes

Main findings

After a single 20-minute in vitro exposure of whole blood to low-intensity SHF radiation at 1000 MHz, intracellular levels of regulatory proteins (notably MDA5) increased in convalescents of community-acquired pneumonia; MAVS and Tmem173 also increased. The exposure increased phosphorylation of the NF-kB inhibitor (IkB-a) and levels of NF-kB components p50 and p65, and was reported to affect interferon-β production; in healthy individuals, MAVS decreased more than MDA5 and Tmem173 while interferon production increased.

Outcomes measured

Intracellular concentration of MAVS
Intracellular concentration of LGP2
Intracellular concentration of MDA5
Intracellular concentration of Tmem173
Intracellular concentration of IRF3
Intracellular concentration of IRF7
Intracellular concentration of IRF8
Intracellular concentration of NF-kB subunits p50 and p65
Phosphorylation of IkB-a (serine 32) and total IkB-a concentration
Spontaneous production of interferon-α/β
Production of interferon-β

Limitations

Sample size not reported in abstract
Exposure intensity/dosimetry (e.g., SAR) not reported
In vitro design; clinical relevance to in vivo exposure not established in abstract
Direction and magnitude of changes for several measured factors not fully specified in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": "in vitro exposure of whole blood (SHF radiation)",
    "frequency_mhz": 1000,
    "sar_wkg": null,
    "duration": "single 20 minutes"
  },
  "population": "Re-convalescents of community-acquired pneumonia and healthy individuals (whole blood samples)",
  "sample_size": null,
  "outcomes": [
    "Intracellular concentration of MAVS",
    "Intracellular concentration of LGP2",
    "Intracellular concentration of MDA5",
    "Intracellular concentration of Tmem173",
    "Intracellular concentration of IRF3",
    "Intracellular concentration of IRF7",
    "Intracellular concentration of IRF8",
    "Intracellular concentration of NF-kB subunits p50 and p65",
    "Phosphorylation of IkB-a (serine 32) and total IkB-a concentration",
    "Spontaneous production of interferon-α/β",
    "Production of interferon-β"
  ],
  "main_findings": "After a single 20-minute in vitro exposure of whole blood to low-intensity SHF radiation at 1000 MHz, intracellular levels of regulatory proteins (notably MDA5) increased in convalescents of community-acquired pneumonia; MAVS and Tmem173 also increased. The exposure increased phosphorylation of the NF-kB inhibitor (IkB-a) and levels of NF-kB components p50 and p65, and was reported to affect interferon-β production; in healthy individuals, MAVS decreased more than MDA5 and Tmem173 while interferon production increased.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure intensity/dosimetry (e.g., SAR) not reported",
    "In vitro design; clinical relevance to in vivo exposure not established in abstract",
    "Direction and magnitude of changes for several measured factors not fully specified in abstract"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave",
    "SHF radiation",
    "1000 MHz",
    "in vitro",
    "whole blood",
    "community-acquired pneumonia",
    "convalescence",
    "innate immunity",
    "antiviral defense",
    "MAVS",
    "MDA5",
    "LGP2",
    "Tmem173",
    "IRF3",
    "IRF7",
    "IRF8",
    "NF-kB",
    "IkB-a",
    "interferon"
  ],
  "suggested_hubs": [
    {
      "slug": "rf-bioeffects",
      "weight": 0.9,
      "reason": "In vitro study of low-intensity microwave (1000 MHz) effects on immune/antiviral signaling proteins and interferon production."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 30601633

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