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Proliferation and cytogenetic studies in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation.

International journal of radiation biology · 1997 · No effect · Low evidence

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No effectLow evidencePaper 4160

No significant differences were observed between 2450 MHz RFR-exposed and sham-exposed lymphocytes for mitotic indices, chromosomal damage measures, binucleate lymphocytes, or micronuclei after either continuous or intermittent exposure. Positive control (150…

Study snapshot

PublicationInternational journal of radiation biology · 1997
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

Aliquots of human peripheral blood collected from two healthy human volunteers were exposed in vitro to continuous wave 2450 MHz radiofrequency radiation (RFR), either continuously for a period of 90 min or intermittently for a total exposure period of 90 min (30 min on and 30 min off, repeated three times). Blood aliquots which were sham-exposed or exposed in vitro to 150 cGy gamma radiation served as controls. The continuous wave 2450 MHz RFR was generated with a net forward power of 34.5 W and transmitted from a standard gain rectangular antenna horn in a vertically downward direction. The mean power density at the position of the cells was 5.0 mW/cm2. The mean specific absorption rate calculated by Finite Difference Time Domain analysis was 12.46 W/kg. Immediately after exposure, lymphocytes were cultured for 48 and 72 h to determine the incidence of chromosomal aberrations and micronuclei, respectively. Proliferation indices were also recorded. There were no significant differences between RFR-exposed and sham-exposed lymphocytes with respect to; (a) mitotic indices; (b) incidence of cells showing chromosome damage; (c) exchange aberrations; (d) acentric fragments; (e) binucleate lymphocytes, and (f) micronuclei, for either the continuous or intermittent RFR exposures. In contrast, the response of positive control cells exposed to 150 cGy gamma radiation was significantly different from RFR-exposed and sham-exposed lymphocytes. Thus, there is no evidence for an effect on mitogen-stimulated proliferation kinetics or for excess genotoxicity within 72 h in human blood lymphocytes exposed in vitro to 2450 MHz RFR.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationHuman peripheral blood lymphocytes from two healthy volunteers (in vitro)
Sample size2
Exposuremicrowave · 2450 MHz · 12.46 W/kg · 90 min (continuous) or intermittent total 90 min (30 min on/30 min off x3)
Evidence strengthLowConfidence: 86% · Peer reviewed: yes

Main findings

No significant differences were observed between 2450 MHz RFR-exposed and sham-exposed lymphocytes for mitotic indices, chromosomal damage measures, binucleate lymphocytes, or micronuclei after either continuous or intermittent exposure. Positive control (150 cGy gamma radiation) showed significant effects compared with RFR and sham.

Outcomes measured

  • Mitotic index
  • Chromosomal aberrations (cells showing chromosome damage, exchange aberrations, acentric fragments)
  • Binucleate lymphocytes
  • Micronuclei
  • Proliferation indices / mitogen-stimulated proliferation kinetics

Limitations

  • Only two human donors were used
  • In vitro study (may not generalize to in vivo exposures)
  • Outcomes assessed only up to 72 h post-exposure
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": 2450,
    "sar_wkg": 12.46,
    "duration": "90 min (continuous) or intermittent total 90 min (30 min on/30 min off x3)"
  },
  "population": "Human peripheral blood lymphocytes from two healthy volunteers (in vitro)",
  "sample_size": 2,
  "outcomes": [
    "Mitotic index",
    "Chromosomal aberrations (cells showing chromosome damage, exchange aberrations, acentric fragments)",
    "Binucleate lymphocytes",
    "Micronuclei",
    "Proliferation indices / mitogen-stimulated proliferation kinetics"
  ],
  "main_findings": "No significant differences were observed between 2450 MHz RFR-exposed and sham-exposed lymphocytes for mitotic indices, chromosomal damage measures, binucleate lymphocytes, or micronuclei after either continuous or intermittent exposure. Positive control (150 cGy gamma radiation) showed significant effects compared with RFR and sham.",
  "effect_direction": "no_effect",
  "limitations": [
    "Only two human donors were used",
    "In vitro study (may not generalize to in vivo exposures)",
    "Outcomes assessed only up to 72 h post-exposure"
  ],
  "evidence_strength": "low",
  "confidence": 0.86,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "2450 MHz",
    "radiofrequency radiation",
    "microwave",
    "in vitro",
    "human blood lymphocytes",
    "chromosomal aberrations",
    "micronuclei",
    "genotoxicity",
    "proliferation",
    "specific absorption rate"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/095530097142915
PubMed: 9416798

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