Stress proteins are not induced in mammalian cells exposed to radiofrequency or microwave radiation.

Bioelectromagnetics · 1997 · No effect · Low evidence

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No effectLow evidencePaper 6340

Study snapshot

PublicationBioelectromagnetics · 1997

Study typein_vitro

Reported directionNo effect

Evidence ratingLow

Abstract

The induction of stress proteins in HeLa and CHO cells was investigated following a 2 h exposure to radiofrequency (RF) or microwave radiation. Cells were exposed or sham exposed in vitro under isothermal (37 +/- 0.2 degrees C) conditions. HeLa cells were exposed to 27- or 2450 MHz continuous wave (CW) radiation at a specific absorption rate (SAR) of 25 W/kg. CHO cells were exposed to CW 27 MHz radiation at a SAR of 100 W/kg. Parallel positive control studies included 2 h exposure of HeLa or CHO cells to 40 degrees C or to 45 microM cadmium sulfate. Stress protein induction was assayed 24 h after treatment by electrophoresis of whole-cell extracted protein labeled with [35S]-methionine. Both cell types exhibited well-characterized responses to the positive control stresses. Under these exposure conditions, neither microwave nor RF radiation had a detectable effect on stress protein induction as determined by either comparison of RF-exposed cells with sham-exposed cells or comparison with heat-stressed or Cd++ positive control cells.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionno_effect

PopulationHeLa and CHO mammalian cells (in vitro)

Sample sizeNot supplied

ExposureRF/microwave · 2 h

Evidence strengthLowConfidence: 86% · Peer reviewed: yes

Main findings

HeLa cells exposed for 2 h to continuous-wave 27 or 2450 MHz radiation at SAR 25 W/kg and CHO cells exposed for 2 h to continuous-wave 27 MHz radiation at SAR 100 W/kg showed no detectable effect on stress protein induction compared with sham exposure under isothermal conditions. Positive controls (40°C heat exposure or 45 µM cadmium sulfate) produced expected stress protein responses in both cell types.

Outcomes measured

Stress protein induction

Limitations

Sample size not reported in abstract.
Only specific cell lines (HeLa, CHO) and exposure conditions (2 h CW; specific frequencies and SARs) were tested.
Outcome assessed at a single post-exposure time point (24 h).

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF/microwave",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "2 h"
  },
  "population": "HeLa and CHO mammalian cells (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Stress protein induction"
  ],
  "main_findings": "HeLa cells exposed for 2 h to continuous-wave 27 or 2450 MHz radiation at SAR 25 W/kg and CHO cells exposed for 2 h to continuous-wave 27 MHz radiation at SAR 100 W/kg showed no detectable effect on stress protein induction compared with sham exposure under isothermal conditions. Positive controls (40°C heat exposure or 45 µM cadmium sulfate) produced expected stress protein responses in both cell types.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in abstract.",
    "Only specific cell lines (HeLa, CHO) and exposure conditions (2 h CW; specific frequencies and SARs) were tested.",
    "Outcome assessed at a single post-exposure time point (24 h)."
  ],
  "evidence_strength": "low",
  "confidence": 0.86,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "HeLa",
    "CHO",
    "in vitro",
    "radiofrequency",
    "microwave",
    "27 MHz",
    "2450 MHz",
    "continuous wave",
    "SAR",
    "25 W/kg",
    "100 W/kg",
    "stress proteins",
    "heat shock proteins",
    "cadmium sulfate",
    "isothermal exposure",
    "sham exposure"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/(sici)1521-186x(1997)18:7<499::aid-bem5>3.0.co;2-y
PubMed: 9338631

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