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[Effect of vitamin E on morphological variation of retinal ganglion cells after microwave radiation].

Wei sheng yan jiu = Journal of hygiene research · 2001 · Mixed · Low evidence

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MixedLow evidencePaper 6380

After 2450 MHz continuous-wave microwave exposure (30 mW/cm2 for 1 h), some cultured pig retinal ganglion cells congregated and axons disappeared; ultrastructural changes included swelling of mitochondria and endoplasmic reticulum, and apoptotic cells were ob…

Study snapshot

PublicationWei sheng yan jiu = Journal of hygiene research · 2001
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

To determine the morphological variation in the primary cultured pig retinal ganglion cells induced by microwave and the protection of VE can supply some experiment foundation for study of effect of microwave and its protection. Retinal ganglion cells of pig were cultured in vitro and added VE of different concentration, Each group was taken after 30 mW/cm2 microwave intensity radiated for 1 h in shielded room by 2450 MHz continuous wave physiotherapy machine. Immediately after radiation, the morphological variation of cells was observed by optics microscope and transmission electronic microscope. The result showed that a portion of cells congregated, with their axon disappeared after radiation. Mitochondria and endoplasmic reticulum are detected swelling by transmission electronic microscope. The results showed that A poptosis cells can be observed. Cells of VE added groups had not obvious changes with optics microscope, but could be found that mitochondria swelling lightly and integrate mitochondria cristae by transmission electronic microscope. The results showed that microwave induced the morphological damage in primary cultured retinal ganglion cells, VE could reduced the damage of retina ganglion cells by microwave in some extent.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationPrimary cultured pig retinal ganglion cells (in vitro)
Sample sizeNot supplied
Exposuremicrowave · 2450 MHz · 1 h
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

After 2450 MHz continuous-wave microwave exposure (30 mW/cm2 for 1 h), some cultured pig retinal ganglion cells congregated and axons disappeared; ultrastructural changes included swelling of mitochondria and endoplasmic reticulum, and apoptotic cells were observed. In vitamin E (VE) groups, no obvious changes were seen by light microscopy, while TEM showed mild mitochondrial swelling and intact mitochondrial cristae; VE was reported to reduce microwave-associated damage to some extent.

Outcomes measured

  • Morphological changes in retinal ganglion cells (light microscopy, transmission electron microscopy)
  • Axon disappearance
  • Mitochondrial swelling
  • Endoplasmic reticulum swelling
  • Apoptotic cells

Limitations

  • In vitro cell culture model (pig retinal ganglion cells)
  • Sample size not reported in abstract
  • Vitamin E concentrations not specified in abstract
  • Exposure metric reported as power density (30 mW/cm2); SAR not reported
  • Outcomes are morphological/ultrastructural observations without quantitative effect estimates in abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": "physiotherapy machine",
    "frequency_mhz": 2450,
    "sar_wkg": null,
    "duration": "1 h"
  },
  "population": "Primary cultured pig retinal ganglion cells (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Morphological changes in retinal ganglion cells (light microscopy, transmission electron microscopy)",
    "Axon disappearance",
    "Mitochondrial swelling",
    "Endoplasmic reticulum swelling",
    "Apoptotic cells"
  ],
  "main_findings": "After 2450 MHz continuous-wave microwave exposure (30 mW/cm2 for 1 h), some cultured pig retinal ganglion cells congregated and axons disappeared; ultrastructural changes included swelling of mitochondria and endoplasmic reticulum, and apoptotic cells were observed. In vitamin E (VE) groups, no obvious changes were seen by light microscopy, while TEM showed mild mitochondrial swelling and intact mitochondrial cristae; VE was reported to reduce microwave-associated damage to some extent.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell culture model (pig retinal ganglion cells)",
    "Sample size not reported in abstract",
    "Vitamin E concentrations not specified in abstract",
    "Exposure metric reported as power density (30 mW/cm2); SAR not reported",
    "Outcomes are morphological/ultrastructural observations without quantitative effect estimates in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "2450 MHz",
    "continuous wave",
    "retinal ganglion cells",
    "pig",
    "in vitro",
    "morphology",
    "mitochondria",
    "endoplasmic reticulum",
    "apoptosis",
    "vitamin E"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 11255758

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