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Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.

International journal of radiation biology · 2021 · Mixed · Low evidence

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MixedLow evidencePaper 5689

AGS cell viability decreased after exposure to 0.2 or 2 mT ELF-EMF compared with control. miR-144 and miR-375 were up-regulated under magnetic field exposure; miR levels were reported to be significantly decreased at 18 and 36 h after exposure ended, but not …

Study snapshot

PublicationInternational journal of radiation biology · 2021
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

PURPOSE: Recently, therapeutic effects of extremely low-frequency electromagnetic field (ELF-EMF) as complementary and alternative medicine, used in the oncology field to control disease symptoms. Micro RNAs (miRs) are responsible for the post-transcriptional regulation of gene expression in the cell. This study aimed to evaluate the expression changes of miR-144 and miR-375 in the human gastric adenocarcinoma cell line (AGS) under the exposure of ELF-EMF. MATERIALS AND METHODS: AGS cells were exposed to magnetic flux densities of 0.2 and 2 mT for 18 h, continuously and discontinuously (1.5 h on/1.5 h off). Cell viability was evaluated by MTT assay. Changes of miR-144 expression levels in AGS cells immediately after exposure and 18 and 36 h after the exposure cut-off was calculated by QRT-PCR. RESULTS: The cell viability of AGS cells was decreased under the exposure of 0.2 and 2 mT EMFs when compared to the control. Up-regulation of miR-144 and miR-375 were observed in AGS cells under the exposure of magnetic fields. CONCLUSIONS: The results indicated that the miR levels were significantly decreased 18 and 36 h after finishing the exposure, but not reached the normal range. The results of this investigation indicated that weak and moderate intermittent 50 Hz ELF-EMFs can induce changes in miRNA expression.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationHuman gastric adenocarcinoma cell line (AGS)
Sample sizeNot supplied
ExposureELF · 0.05 MHz · 18 h (continuous or intermittent 1.5 h on/1.5 h off)
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

AGS cell viability decreased after exposure to 0.2 or 2 mT ELF-EMF compared with control. miR-144 and miR-375 were up-regulated under magnetic field exposure; miR levels were reported to be significantly decreased at 18 and 36 h after exposure ended, but not back to the normal range.

Outcomes measured

  • Cell viability (MTT assay)
  • miR-144 expression (qRT-PCR)
  • miR-375 expression

Limitations

  • In vitro cell-line study; findings may not generalize to humans or clinical outcomes
  • Sample size not reported in abstract
  • Exposure source/setup details beyond flux density and frequency not provided in abstract
  • Only two miRNAs assessed; limited scope of molecular endpoints
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "18 h (continuous or intermittent 1.5 h on/1.5 h off)"
  },
  "population": "Human gastric adenocarcinoma cell line (AGS)",
  "sample_size": null,
  "outcomes": [
    "Cell viability (MTT assay)",
    "miR-144 expression (qRT-PCR)",
    "miR-375 expression"
  ],
  "main_findings": "AGS cell viability decreased after exposure to 0.2 or 2 mT ELF-EMF compared with control. miR-144 and miR-375 were up-regulated under magnetic field exposure; miR levels were reported to be significantly decreased at 18 and 36 h after exposure ended, but not back to the normal range.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell-line study; findings may not generalize to humans or clinical outcomes",
    "Sample size not reported in abstract",
    "Exposure source/setup details beyond flux density and frequency not provided in abstract",
    "Only two miRNAs assessed; limited scope of molecular endpoints"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "magnetic field",
    "0.2 mT",
    "2 mT",
    "AGS",
    "gastric adenocarcinoma",
    "cell viability",
    "MTT",
    "miR-144",
    "miR-375",
    "miRNA expression",
    "intermittent exposure"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Study evaluates 50 Hz extremely low-frequency electromagnetic fields in vitro."
    },
    {
      "slug": "cancer-research",
      "weight": 0.55,
      "reason": "Uses a human gastric cancer cell line and assesses viability and miRNA expression changes."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/09553002.2021.1941376
PubMed: 34125651

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