Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis

Iran J Cancer Prev · 2014 · Mixed · Low evidence

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MixedLow evidencePaper 2758

SH-SY5Y cells exposed to a 2 mT, 50 Hz magnetic field for 3 hours showed changes in expression of 189 proteins relative to controls based on 2D electrophoresis analysis. PCA/clustering indicated protein data clustered by experimental condition, and the author…

Study snapshot

PublicationIran J Cancer Prev · 2014

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Background: Extremely low frequency electromagnetic fields (ELF-EMF) have been common in daily life all over the world. They have produced by power lines and electrical appliances, but higher levels of them have raised a lot of concerns about their carcinogenesis. Both epidemiological and laboratory studies have suggested that EMFs might increase cancer incidence, including acute childhood leukemia, brain and breast cancer. Methods: In the present study, SH-SY5Y human neuroblastoma cell line has exposed to 2mT, 50 Hz magnetic field for 3 h. Next, effect of this exposure on protein expression including over-expression or under-expression has assessed by proteomics. Results: Bioinformatics and statistical analysis using progenesis same spot software on the obtained 2D electrophoresis has shown that expression of 189 proteins in exposed group has changed relative to control. Besides, PCA analysis has verified results of clustering, and has shown that protein data has clustered according to experimental conditions. Conclusion: The results of this study have shown that ELF-EMF changes cell morphology via altering protein expression, but more profound studies have needed to determine the kind of proteins altered.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationSH-SY5Y human neuroblastoma cell line

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 3 h

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Protein expression changes (proteomics; 2D electrophoresis)
Cell morphology changes

Limitations

In vitro cell-line study (human neuroblastoma cells)
Sample size not reported in abstract
Specific proteins altered not determined/characterized in the abstract
Exposure limited to a single field strength (2 mT), frequency (50 Hz), and duration (3 h)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "power lines and electrical appliances",
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "3 h"
  },
  "population": "SH-SY5Y human neuroblastoma cell line",
  "sample_size": null,
  "outcomes": [
    "Protein expression changes (proteomics; 2D electrophoresis)",
    "Cell morphology changes"
  ],
  "main_findings": "SH-SY5Y cells exposed to a 2 mT, 50 Hz magnetic field for 3 hours showed changes in expression of 189 proteins relative to controls based on 2D electrophoresis analysis. PCA/clustering indicated protein data clustered by experimental condition, and the authors conclude ELF-EMF changes cell morphology via altered protein expression.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell-line study (human neuroblastoma cells)",
    "Sample size not reported in abstract",
    "Specific proteins altered not determined/characterized in the abstract",
    "Exposure limited to a single field strength (2 mT), frequency (50 Hz), and duration (3 h)"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "extremely low frequency",
    "50 Hz",
    "2 mT",
    "magnetic field",
    "SH-SY5Y",
    "human neuroblastoma",
    "proteomics",
    "2D electrophoresis",
    "PCA",
    "protein expression",
    "cell morphology"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "Background mentions ELF-EMF from power lines/electrical appliances; no specific occupational setting studied."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 25250144

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