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EVALUATION OF BCL2 AND ITS REGULATORY MIRS, MIR-15-B AND MIR-16 EXPRESSION CHANGES UNDER THE EXPOSURE OF EXTREMELY LOW-FREQUENCY ELECTROMAGNETIC FIELDS ON HUMAN GASTRIC CANCER CELL LINE.

Radiation protection dosimetry · 2021 · Mixed · Low evidence

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MixedLow evidencePaper 5667

ELF-EMF exposure at 2 mT continuous significantly reduced AGS cell viability. BCL2 expression was significantly decreased after continuous exposure at 0.2 and 2 mT and after discontinuous exposure at 2 mT. miR-15-b and miR-16 expression were significantly inc…

Study snapshot

PublicationRadiation protection dosimetry · 2021
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

In this research, changes in the expression of B-cell lymphoma 2 (BCL2), miR-15-b and miR-16 in human adenocarcinoma gastric cancer cell line (AGS) following the exposure to magnetic flux densities (MFDs) of 0.2 and 2 mT continuously and discontinuously (1.5 h on/1.5 h off) for 18 h were investigated. Changes in the cell viability were evaluated by the MTT assay. Real-time PCR was used to evaluate the expression changes of BCL2, miR-15-b and miR-16. The results showed that extremely low frequency electromagnetic field (ELF-EMF) could significantly reduce the viability of AGS cells in the continuous MFD of 2 mT. The BCL2 expression was significantly decreased following the exposure to continuous MFDs of 0.2 and 2 mT and discontinuous MFD of 2 mT. The expressions of miR-15-b and miR-16 were significantly increased in continuous and discontinuous MFD of 2 mT. According to the results, weak and moderate extremely low-frequency electromagnetic fields can change the expressions of BCL2, miR-15-b and miR-16.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationHuman gastric adenocarcinoma cancer cell line (AGS)
Sample sizeNot supplied
ExposureELF · 18 h (continuous or discontinuous 1.5 h on/1.5 h off)
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

ELF-EMF exposure at 2 mT continuous significantly reduced AGS cell viability. BCL2 expression was significantly decreased after continuous exposure at 0.2 and 2 mT and after discontinuous exposure at 2 mT. miR-15-b and miR-16 expression were significantly increased in both continuous and discontinuous 2 mT conditions.

Outcomes measured

  • Cell viability (MTT assay)
  • BCL2 expression (real-time PCR)
  • miR-15-b expression (real-time PCR)
  • miR-16 expression (real-time PCR)

Limitations

  • Frequency of the ELF-EMF exposure was not reported in the abstract.
  • In vitro cancer cell line model; generalizability to humans is not addressed in the abstract.
  • Sample size/replicates and detailed dosimetry beyond magnetic flux density are not provided in the abstract.
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "18 h (continuous or discontinuous 1.5 h on/1.5 h off)"
  },
  "population": "Human gastric adenocarcinoma cancer cell line (AGS)",
  "sample_size": null,
  "outcomes": [
    "Cell viability (MTT assay)",
    "BCL2 expression (real-time PCR)",
    "miR-15-b expression (real-time PCR)",
    "miR-16 expression (real-time PCR)"
  ],
  "main_findings": "ELF-EMF exposure at 2 mT continuous significantly reduced AGS cell viability. BCL2 expression was significantly decreased after continuous exposure at 0.2 and 2 mT and after discontinuous exposure at 2 mT. miR-15-b and miR-16 expression were significantly increased in both continuous and discontinuous 2 mT conditions.",
  "effect_direction": "mixed",
  "limitations": [
    "Frequency of the ELF-EMF exposure was not reported in the abstract.",
    "In vitro cancer cell line model; generalizability to humans is not addressed in the abstract.",
    "Sample size/replicates and detailed dosimetry beyond magnetic flux density are not provided in the abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "extremely low frequency",
    "magnetic flux density",
    "0.2 mT",
    "2 mT",
    "AGS",
    "gastric cancer cell line",
    "cell viability",
    "MTT",
    "BCL2",
    "miR-15-b",
    "miR-16",
    "gene expression",
    "real-time PCR"
  ],
  "suggested_hubs": []
}
AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1093/rpd/ncab163
PubMed: 34791478

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