The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells.

Acta odontologica Scandinavica · 2020 · Benefit · Low evidence

← Research stream

BenefitLow evidencePaper 5646

Exposure to 50 Hz ELF-EMF at 0.5 or 1 mT for 20 or 40 min/day over 7 days was associated with significantly higher survival and proliferation in all exposure groups compared with control. The highest viable cell number/proliferation was reported for 1 mT at 2…

Study snapshot

PublicationActa odontologica Scandinavica · 2020

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

Extremely low-frequency electromagnetic fields (ELF-EMF) can affect biological systems and alter some cell functions like proliferation rate. Dental pulp tissue is known as a source of multipotent stromal stem cells (MSCs), which can be obtained by a less invasive and more available process compared to bone marrow-derived stem cells (BMSCs). This study aimed to consider the effect of ELF-EMF on proliferation rates of human dental pulp stem cells (hDPSCs). ELF-EMF was generated by a system including autotransformer, multi-meter, solenoid coils, teslameter and its probe. The effect of ELF-EMF with the intensity of 0.5 and 1 mT and 50 Hz on the proliferation rate of hDPSCs was assessed in 20 and 40 min per day for 7 days. MTT assay and DAPI test were used to determine the growth and proliferation of DPSCs. Based on MTT, ELF-EMF has maximum effect with the intensity of 1 mT for 20 min/day on the proliferation of hDPSCs. The survival and proliferation rate in all exposure groups were significantly higher than the control group. Based on the data obtained from MTT and DAPI assay, the number of viable cells in the group exposed to 1 mT for 20 min/day was higher than other groups ( < .05). Regarding to the results of this study, 0.5 and 1 mT ELF-EMF can enhance survival and proliferation rates of hDPSCs.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationHuman dental pulp stem cells (hDPSCs)

Sample sizeNot supplied

ExposureELF · 5e-05 MHz · 20 or 40 min/day for 7 days

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Cell survival
Cell proliferation/growth (MTT assay)
Viable cell number (DAPI assay)

Limitations

In vitro study (human cells in culture)
No sample size reported in abstract
No SAR or dosimetry beyond field intensity/frequency described in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "solenoid coils exposure system",
    "frequency_mhz": 5e-05,
    "sar_wkg": null,
    "duration": "20 or 40 min/day for 7 days"
  },
  "population": "Human dental pulp stem cells (hDPSCs)",
  "sample_size": null,
  "outcomes": [
    "Cell survival",
    "Cell proliferation/growth (MTT assay)",
    "Viable cell number (DAPI assay)"
  ],
  "main_findings": "Exposure to 50 Hz ELF-EMF at 0.5 or 1 mT for 20 or 40 min/day over 7 days was associated with significantly higher survival and proliferation in all exposure groups compared with control. The highest viable cell number/proliferation was reported for 1 mT at 20 min/day (p<.05).",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study (human cells in culture)",
    "No sample size reported in abstract",
    "No SAR or dosimetry beyond field intensity/frequency described in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "0.5 mT",
    "1 mT",
    "dental pulp stem cells",
    "hDPSCs",
    "proliferation",
    "survival",
    "MTT",
    "DAPI"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Study evaluates extremely low-frequency EMF (50 Hz) effects on cells."
    }
  ]
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/00016357.2020.1734655
PubMed: 32191156

Read the original paper →

Related papers

← Back to the Research Library