Proteomic Analysis of the Effect of ELF-EMF With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

2017 · Harm · Low evidence

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HarmLow evidencePaper 2255

SH-SY5Y cells exposed to 50 Hz ELF-EMF at 0.5 or 1 mT for 3 hours showed changes in cell morphology and a dose-dependent decrease in proliferation rate. Proteomic/bioinformatic analyses indicated altered protein expression with exposure, described as dose-dep…

Study snapshot

Publicationmanual · 2017

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

Proteomic Analysis of the Effect of ELF-EMF With Different Intensities in SH-SY5Y Neuroblastoma Cell Line Rezaie-Tavirani M, Hasanzadeh H, Seyyedi S, Zali H.Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line.J Lasers Med Sci. 2017 Spring;8(2):79-83. doi: 10.15171/jlms.2017.14. Abstract Introduction: During the last 3 decades, human is exposed to extremely low frequency electromagnetic fields (ELF-EMF) emitted by power lines and electronic devices. It is now well accepted that ELF-EMF are able to produce a variety of biological effects, although the molecular mechanism is unclear and controversial. Investigation of different intensities effects of 50 Hz ELF-EMF on cell morphology and protein expression is the aim of this study. Methods: SH-SY5Y human neuroblastoma cell line was exposed to 0.5 and 1 mT 50 Hz (ELF-EMF) for 3 hours. Proteomics techniques were used to determine the effects of these fields on protein expression. Bioinformatic and statistical analysis of proteomes were performed using Progensis SameSpots software. Results: Our results showed that exposure to ELF-EMF changes cell morphology and induces a dose-dependent decrease in the proliferation rate of the cells. The proteomic studies and bioinformatic analysis indicate that exposure to 50 Hz ELF-EMF leads to alteration of cell protein expression in both dose-dependent and intensity dependent manner, but the later is more pronounced. Conclusion: Our data suggests that increased intensity of ELF-EMF may be associated with more alteration in cell protein expression, as well as effect on cell morphology and proliferation. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationSH-SY5Y human neuroblastoma cell line

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 3 hours

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Cell morphology
Cell proliferation rate
Protein expression (proteomics)

Limitations

In vitro cell line study; applicability to humans not addressed in abstract
Sample size/replicates not reported in abstract
Exposure duration limited to 3 hours

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "power lines and electronic devices",
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "3 hours"
  },
  "population": "SH-SY5Y human neuroblastoma cell line",
  "sample_size": null,
  "outcomes": [
    "Cell morphology",
    "Cell proliferation rate",
    "Protein expression (proteomics)"
  ],
  "main_findings": "SH-SY5Y cells exposed to 50 Hz ELF-EMF at 0.5 or 1 mT for 3 hours showed changes in cell morphology and a dose-dependent decrease in proliferation rate. Proteomic/bioinformatic analyses indicated altered protein expression with exposure, described as dose-dependent and intensity-dependent, with intensity effects reported as more pronounced.",
  "effect_direction": "harm",
  "limitations": [
    "In vitro cell line study; applicability to humans not addressed in abstract",
    "Sample size/replicates not reported in abstract",
    "Exposure duration limited to 3 hours"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "0.5 mT",
    "1 mT",
    "SH-SY5Y",
    "neuroblastoma",
    "proteomics",
    "protein expression",
    "cell morphology",
    "proliferation"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.25,
      "reason": "Mentions ELF-EMF from power lines/electronic devices; general relevance to common/occupational ELF sources."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.15171/jlms.2017.14
PubMed: Not supplied

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