Extremely low-frequency electromagnetic field influences the survival and proliferation effect of human adipose derived stem cells.

Advanced biomedical research · 2014 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 4703

Exposure to 50 Hz ELF-EMF at 0.5 or 1 mT for 20 or 40 min/day over 7 days generally increased hADSC survival and proliferation effect compared with sham (P < 0.05), except for the 1 mT, 40 min/day group. The maximum reported effect was at 1 mT for 20 min/day.

Study snapshot

PublicationAdvanced biomedical research · 2014

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

BACKGROUND: Extremely low-frequency electromagnetic fields (ELF-EMF) can effect on biological systems and alters some cell functions like proliferation rate. Therefore, we aimed to attempt the evaluation effect of ELF-EMF on the growth of human adipose derived stem cells (hADSCs). MATERIALS AND METHODS: ELF-EMF was generated by a system including autotransformer, multi-meter, solenoid coils, teslameter and its probe. We assessed the effect of ELF-EMF with intensity of 0.5 and 1 mT and power line frequency 50 Hz on the survival of hADSCs for 20 and 40 min/day for 7 days by MTT assay. One-way analysis of variance was used to assessment the significant differences in groups. RESULTS: ELF-EMF has maximum effect with intensity of 1 mT for 20 min/day on proliferation of hADSCs. The survival and proliferation effect (PE) in all exposure groups were significantly higher than that in sham groups (P < 0.05) except in group of 1 mT and 40 min/day. CONCLUSION: Our results show that between 0.5 m and 1 mT ELF-EMF could be enhances survival and PE of hADSCs conserving the duration of exposure.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman adipose derived stem cells (hADSCs)

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 20 or 40 min/day for 7 days

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Cell survival (MTT assay)
Cell proliferation / proliferation effect (PE)

Limitations

In vitro study (cell culture), not directly generalizable to humans
Sample size not reported in abstract
Exposure characterization limited to frequency, intensity, and duration; other parameters not described
One exposure condition (1 mT, 40 min/day) did not show significant increase vs sham

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "20 or 40 min/day for 7 days"
  },
  "population": "Human adipose derived stem cells (hADSCs)",
  "sample_size": null,
  "outcomes": [
    "Cell survival (MTT assay)",
    "Cell proliferation / proliferation effect (PE)"
  ],
  "main_findings": "Exposure to 50 Hz ELF-EMF at 0.5 or 1 mT for 20 or 40 min/day over 7 days generally increased hADSC survival and proliferation effect compared with sham (P < 0.05), except for the 1 mT, 40 min/day group. The maximum reported effect was at 1 mT for 20 min/day.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study (cell culture), not directly generalizable to humans",
    "Sample size not reported in abstract",
    "Exposure characterization limited to frequency, intensity, and duration; other parameters not described",
    "One exposure condition (1 mT, 40 min/day) did not show significant increase vs sham"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "0.5 mT",
    "1 mT",
    "human adipose derived stem cells",
    "hADSCs",
    "proliferation",
    "survival",
    "MTT assay"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.4103/2277-9175.124668
PubMed: 24592372

Read the original paper →

Related papers

← Back to the Research Library