Different profiles of the mRNA levels of DNA repair genes in MCF-7 and SH-SY5Y cells after treatment with combination of cisplatin, 50-Hz electromagnetic field and bleomycin.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2017 · Mixed · Very low evidence

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MixedVery low evidencePaper 4912

MCF-7 cells treated with CDDP+EMF+Bleo showed more susceptibility than cells treated with CDDP+Bleo, while SH-SY5Y susceptibility was not changed between these two treatments. The two cell lines showed non-random disagreement in DNA repair gene expression in …

Study snapshot

PublicationBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2017

Study typein_vitro

Reported directionMixed

Evidence ratingVery low

Abstract

Neurotoxicity is known to be a major dose-limiting adverse effect of cisplatin (CDDP), alone or in combination with other chemicals. DNA repair capacity serve as a neuroprotective factor against CDDP. The purpose of this study was to evaluate the effect of 50-Hz electromagnetic field (EMF) in combination with CDDP and bleomycin (Bleo) on expression of some of DNA repair genes (GADD45A, XRCC1, XRCC4, Ku70, Ku80, DNA-PKcs and LIG4) in MCF-7 (breast cancer) and SH-SY5Y (neuroblastoma) cell lines. MCF-7 and SH-SY5Y cells were pre-treated with CDDP in the presence or absence of EMF and then exposed to different concentration of Bleo. EMF (0.50mT intensity) was used in the intermittenet pattern of "15min field on/15min field off" with 30min total exposure. Cell viability assay was done and then the transcript levels of the examined genes were measured using quantitative real-time PCR in "CDDP+Bleo" and "CDDP+EMF+Bleo" treatments. Our results indicated that MCF-7 cells treated with "CDDP+EMF+Bleo" showed more susceptibility compared with "CDDP+Bleo" treated ones, while SH-SY5Y susceptibility was not changed between the two treatments. The represented data indicated that MCF-7 and SH-SY5Y cells showed non-random disagreement in DNA repair gene expression in 11 conditions (out of 14 conditions) with each other (χ=4.52, df=1, P=0.033). This finding can be promising for sensitizing breast cancer cells while protecting against CDDP induced neuropathy in cancer patients.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationMCF-7 (breast cancer) and SH-SY5Y (neuroblastoma) cell lines

Sample sizeNot supplied

ExposureELF · 0.05 MHz · Intermittent pattern 15 min on/15 min off; 30 min total exposure; 0.50 mT intensity

Evidence strengthVery lowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Cell viability/susceptibility under treatments (CDDP+Bleo vs CDDP+EMF+Bleo)
mRNA/transcript levels of DNA repair genes (GADD45A, XRCC1, XRCC4, Ku70, Ku80, DNA-PKcs, LIG4) measured by quantitative real-time PCR
Disagreement in DNA repair gene expression profiles between MCF-7 and SH-SY5Y across conditions (chi-square test)

Limitations

In vitro cell-line study; clinical relevance not established in abstract
Exposure parameters beyond frequency, intensity, and intermittent schedule not described (e.g., field configuration)
Sample size/replicates not reported in abstract
Outcomes limited to viability and mRNA levels; no protein/function measures described

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "Intermittent pattern 15 min on/15 min off; 30 min total exposure; 0.50 mT intensity"
  },
  "population": "MCF-7 (breast cancer) and SH-SY5Y (neuroblastoma) cell lines",
  "sample_size": null,
  "outcomes": [
    "Cell viability/susceptibility under treatments (CDDP+Bleo vs CDDP+EMF+Bleo)",
    "mRNA/transcript levels of DNA repair genes (GADD45A, XRCC1, XRCC4, Ku70, Ku80, DNA-PKcs, LIG4) measured by quantitative real-time PCR",
    "Disagreement in DNA repair gene expression profiles between MCF-7 and SH-SY5Y across conditions (chi-square test)"
  ],
  "main_findings": "MCF-7 cells treated with CDDP+EMF+Bleo showed more susceptibility than cells treated with CDDP+Bleo, while SH-SY5Y susceptibility was not changed between these two treatments. The two cell lines showed non-random disagreement in DNA repair gene expression in 11 of 14 conditions (χ=4.52, df=1, P=0.033).",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell-line study; clinical relevance not established in abstract",
    "Exposure parameters beyond frequency, intensity, and intermittent schedule not described (e.g., field configuration)",
    "Sample size/replicates not reported in abstract",
    "Outcomes limited to viability and mRNA levels; no protein/function measures described"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50-Hz electromagnetic field",
    "ELF-EMF",
    "cisplatin",
    "bleomycin",
    "DNA repair genes",
    "GADD45A",
    "XRCC1",
    "XRCC4",
    "Ku70",
    "Ku80",
    "DNA-PKcs",
    "LIG4",
    "MCF-7",
    "SH-SY5Y",
    "cell viability",
    "qRT-PCR"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.biopha.2017.07.115
PubMed: 28780472

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