[Synergistic inhibitory effect of static magnetic field and antitumor drugs on Hepa1-6 cells].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology · 2015 · Benefit · Low evidence

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BenefitLow evidencePaper 2952

Static magnetic field (SMF) combined with cisplatin or adriamycin significantly inhibited Hepa1-6 cell growth versus treatment described in the abstract. SMF appeared to enhance drug-associated cell cycle arrest and DNA damage, and combined treatment was asso…

Study snapshot

PublicationSheng wu gong cheng xue bao = Chinese journal of biotechnology · 2015

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

Chemotherapy as a routine method for clinical treatment of cancer has disadvantages such as significant toxicity and strong resistance. In order to improve the efficacy of the drugs and reduce the by-effects, we tried to combine static magnetic field (SMF) with cisplatin or adriamycin. The growth of Hepa1-6 cells treated with the static magnetic field (SMF) combined with cisplatin or adriamycin was significantly inhibited, as detected with MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) test. Combined treatment group cells underwent significant morphological changes as observed by HE (Hematoxylin and eosin) staining under optical microscope. Cell cycle analysis indicated that SMF increased the ratio of cells arrested in G2/M phase caused by cisplatin, and when treated with SMF combined with adriamycin, cells were almost arrested in G1 and G2/M phase. SCGE test showed that SMF can enhance the ability of cisplatin or adriamycin to promote cell DNA damage. Atomic force microscope observation found that the combination of antitumor drugs and magnetic field treatment induced larger and deeper holes on the cell membrane, and surface structure damage is serious. The combination of antitumor drugs and magnetic field technology effectively inhibits the growth of tumor cells, and reduces drug doses. The results implicate this method as potential cancer therapy.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationHepa1-6 cells (mouse hepatoma cell line)

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Cell growth/viability (MTT assay)
Cell morphology (HE staining)
Cell cycle distribution/arrest (flow cytometry)
DNA damage (SCGE/comet assay)
Cell membrane/surface structural damage (atomic force microscopy)

Limitations

In vitro study in a single cell line; clinical relevance not established in abstract
SMF exposure parameters (field strength, duration) not reported in abstract
Drug doses and comparator conditions not detailed in abstract
Sample size/replication not reported in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "static magnetic field",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Hepa1-6 cells (mouse hepatoma cell line)",
  "sample_size": null,
  "outcomes": [
    "Cell growth/viability (MTT assay)",
    "Cell morphology (HE staining)",
    "Cell cycle distribution/arrest (flow cytometry)",
    "DNA damage (SCGE/comet assay)",
    "Cell membrane/surface structural damage (atomic force microscopy)"
  ],
  "main_findings": "Static magnetic field (SMF) combined with cisplatin or adriamycin significantly inhibited Hepa1-6 cell growth versus treatment described in the abstract. SMF appeared to enhance drug-associated cell cycle arrest and DNA damage, and combined treatment was associated with more severe membrane/surface structural damage by atomic force microscopy.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study in a single cell line; clinical relevance not established in abstract",
    "SMF exposure parameters (field strength, duration) not reported in abstract",
    "Drug doses and comparator conditions not detailed in abstract",
    "Sample size/replication not reported in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "static magnetic field",
    "SMF",
    "cisplatin",
    "adriamycin",
    "doxorubicin",
    "Hepa1-6",
    "cell cycle arrest",
    "DNA damage",
    "comet assay",
    "atomic force microscopy",
    "cancer therapy"
  ],
  "suggested_hubs": [
    {
      "slug": "static-magnetic-fields",
      "weight": 0.9,
      "reason": "Study evaluates biological effects of a static magnetic field exposure in vitro."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 26955714

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