Mesenchymal stem cells that located in the electromagnetic fields improves rat model of Parkinson's disease.

Iranian journal of basic medical sciences · 2016 · Benefit · Low evidence

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BenefitLow evidencePaper 4826

Study snapshot

PublicationIranian journal of basic medical sciences · 2016

Study typeanimal

Reported directionBenefit

Evidence ratingLow

Abstract

OBJECTIVES: The main characteristic of mesenchymal stem cells (MSCs) is their ability to produce other cell types. Electromagnetic field (EMF) stimulates differentiation of MSCs into other cells. In this study, we investigated whether EMF can effect on the differentiation of MSCs into dopaminergic (DA) neurons. MATERIALS AND METHODS: An EMF with a frequency of 50 Hz and two intensities of 40 and 400 µT 1hr/day was generated around the cells for a week. Afterwards, these cells were injected into the left ventricle of Parkinsonian rats. The rats survived for 2 weeks, and then sampling was performed. RESULTS: The injected cells differentiated into DA neurons and sporadically settled in the substantia nigra pars compacta (SNpc). Transplanted rats exhibited significant partial correction apomorphine-induced rotational behavior compared to Parkinsonian rats (5.0±0.1 vs 7.57±0.08). Results demonstrated that endogenous serum and brain derived neurotrophic factor (BDNF) were altered in all experimental groups. The greatest increase was in group of 400 µT EMF in comparison with Parkinsonian rats (398±15 vs. 312±11.79 pg ⁄ mg). Current study have shown that 6-Hydroxydopamine can cause severe loss of dopaminergic neurons (68±6.58), but injected MSCs that exposed to 40 and 400 µT EMF increased dopaminergic neurons in SNpc (108±2.33 & 126±3.89) (P<0.001). CONCLUSION: Electromagnetic fields with particular frequencies stimulate MSCs. So, these cells had anti-Parkinsonian properties in our studies.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionbenefit

PopulationParkinsonian rat model (6-hydroxydopamine lesion) receiving mesenchymal stem cells exposed to EMF

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 1 hr/day for 1 week (cell exposure); rats followed for 2 weeks after injection

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

MSCs exposed to 50 Hz EMF (40 or 400 µT, 1 hr/day for 1 week) and then injected into Parkinsonian rats differentiated into dopaminergic neurons and were observed sporadically in the SNpc. Transplanted rats showed partial improvement in apomorphine-induced rotational behavior versus Parkinsonian rats, and dopaminergic neuron counts in SNpc were higher in EMF-exposed MSC groups; BDNF levels were altered across experimental groups with the greatest increase reported in the 400 µT group versus Parkinsonian rats.

Outcomes measured

Differentiation of injected MSCs into dopaminergic neurons
Localization/settlement of injected cells in substantia nigra pars compacta (SNpc)
Apomorphine-induced rotational behavior
Serum and brain BDNF levels
Dopaminergic neuron counts in SNpc

Limitations

Sample size not reported in abstract
Details of randomization/blinding not reported in abstract
Exposure described for cells; in vivo EMF exposure of rats not described
Outcome reporting indicates multiple experimental groups but group definitions and full comparisons are not fully specified in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "1 hr/day for 1 week (cell exposure); rats followed for 2 weeks after injection"
  },
  "population": "Parkinsonian rat model (6-hydroxydopamine lesion) receiving mesenchymal stem cells exposed to EMF",
  "sample_size": null,
  "outcomes": [
    "Differentiation of injected MSCs into dopaminergic neurons",
    "Localization/settlement of injected cells in substantia nigra pars compacta (SNpc)",
    "Apomorphine-induced rotational behavior",
    "Serum and brain BDNF levels",
    "Dopaminergic neuron counts in SNpc"
  ],
  "main_findings": "MSCs exposed to 50 Hz EMF (40 or 400 µT, 1 hr/day for 1 week) and then injected into Parkinsonian rats differentiated into dopaminergic neurons and were observed sporadically in the SNpc. Transplanted rats showed partial improvement in apomorphine-induced rotational behavior versus Parkinsonian rats, and dopaminergic neuron counts in SNpc were higher in EMF-exposed MSC groups; BDNF levels were altered across experimental groups with the greatest increase reported in the 400 µT group versus Parkinsonian rats.",
  "effect_direction": "benefit",
  "limitations": [
    "Sample size not reported in abstract",
    "Details of randomization/blinding not reported in abstract",
    "Exposure described for cells; in vivo EMF exposure of rats not described",
    "Outcome reporting indicates multiple experimental groups but group definitions and full comparisons are not fully specified in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic field",
    "ELF-EMF",
    "50 Hz",
    "microtesla",
    "mesenchymal stem cells",
    "dopaminergic neurons",
    "Parkinson's disease",
    "6-hydroxydopamine",
    "substantia nigra pars compacta",
    "BDNF",
    "apomorphine-induced rotation"
  ],
  "suggested_hubs": [
    {
      "slug": "animal-studies",
      "weight": 0.9,
      "reason": "Parkinsonian rat model with EMF-exposed MSC transplantation"
    },
    {
      "slug": "elf-emf",
      "weight": 0.85,
      "reason": "50 Hz exposure at 40 and 400 µT"
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 27635198

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