Electromagnetic field change the expression of osteogenesis genes in murine bone marrow mesenchymal stem cells.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2008 · Mixed · Very low evidence

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MixedVery low evidencePaper 5012

Osteogenesis microarray analysis identified 19 differentially expressed genes between EMF-stimulated MSCs and controls (6 up-regulated, 13 down-regulated). Semi-quantitative RT-PCR confirmed increased Bmp1 and Bmp7 mRNA and decreased Egf and Egfr mRNA in the …

Study snapshot

PublicationJournal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2008

Study typein_vitro

Reported directionMixed

Evidence ratingVery low

Abstract

In order to identify the differentially expressing gene of bone marrow mesenchymal stem cells (MSCs) stimulated by electromagnetic field (EMF) with osteogenesis microarray analysis, the bone marrow MSCs of SD rats were isolated and cultured in vitro. The third-passage cells were stimulated by EMFs and total RNA was extracted, purified and then used for the synthesis of cDNA and cRNA. The cRNA of stimulated group and the control group was hybridized with the rat oligo osteogenesis microarray respectively. The hybridization signals were acquired by using X-ray film after chemiluminescent detection and the data obtained were analyzed by employing the web-based completely integrated GEArray Expression Analysis Suite. RT-PCR was used to identify the target genes: Bmp1, Bmp7, Egf and Egfr. The results showed that 19 differentially expressing genes were found between the stimulated group and the control group. There were 6 up-regulated genes and 13 down-regulated genes in the stimulated group. Semi-quantitative RT-PCR confirmed that the expressions of Bmp1, Bmp7 mRNA of the stimulated group were up-regulated (P<0.05) and those of Egf, Egfr were down-regulated (P<0.05). It was suggested that the gene expression profiles of osteogenesis of the bone marrow MSCs were changed after EMF treatment. It is concluded that the genes are involved in skeletal development, bone mineral metabolism, cell growth and differentiation, cell adhesion etc.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationBone marrow mesenchymal stem cells (MSCs) isolated from SD rats; third-passage cells cultured in vitro

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthVery lowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Differential expression of osteogenesis-related genes (rat oligo osteogenesis microarray) after EMF stimulation
RT-PCR validation of Bmp1, Bmp7, Egf, Egfr mRNA expression changes

Limitations

EMF exposure parameters (e.g., frequency, intensity, duration) not reported in the abstract
In vitro study; findings may not generalize to in vivo outcomes
Sample size and replication details not provided in the abstract
Outcome limited to gene expression changes; no functional osteogenesis endpoints described in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Bone marrow mesenchymal stem cells (MSCs) isolated from SD rats; third-passage cells cultured in vitro",
  "sample_size": null,
  "outcomes": [
    "Differential expression of osteogenesis-related genes (rat oligo osteogenesis microarray) after EMF stimulation",
    "RT-PCR validation of Bmp1, Bmp7, Egf, Egfr mRNA expression changes"
  ],
  "main_findings": "Osteogenesis microarray analysis identified 19 differentially expressed genes between EMF-stimulated MSCs and controls (6 up-regulated, 13 down-regulated). Semi-quantitative RT-PCR confirmed increased Bmp1 and Bmp7 mRNA and decreased Egf and Egfr mRNA in the EMF-stimulated group (P<0.05).",
  "effect_direction": "mixed",
  "limitations": [
    "EMF exposure parameters (e.g., frequency, intensity, duration) not reported in the abstract",
    "In vitro study; findings may not generalize to in vivo outcomes",
    "Sample size and replication details not provided in the abstract",
    "Outcome limited to gene expression changes; no functional osteogenesis endpoints described in the abstract"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic field",
    "EMF",
    "mesenchymal stem cells",
    "bone marrow",
    "osteogenesis",
    "microarray",
    "gene expression",
    "Bmp1",
    "Bmp7",
    "Egf",
    "Egfr",
    "rat"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s11596-008-0209-3
PubMed: 18480985

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