Exposure to extremely low-frequency electromagnetic fields inhibits T-type calcium channels via AA/LTE4 signaling pathway.

Cell calcium · 2014 · Mixed · Low evidence

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MixedLow evidencePaper 4555

Study snapshot

PublicationCell calcium · 2014

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Extremely low-frequency electromagnetic fields (ELF-EMF) causes various biological effects through altering intracellular calcium homeostasis. The role of high voltage-gated (HVA) calcium channels in ELF-EMF induced effects has been extensively studied. However, the effect of ELF-EMF on low-voltage-gated (LVA) T-type calcium channels has not been reported. In this study, we test the effect of ELF-EMF (50Hz) on human T-type calcium channels transfected in HEK293 cells. Conversely to its stimulant effects on HVA channels, ELF-EMF exposure inhibited all T-type (Cav3.1, Cav3.2 and Cav3.3) channels. Neither the protein expression nor the steady-state activation and inactivation kinetics of Cav3.2 channels were altered by ELF-EMF (50Hz, 0.2mT) exposure. Exposure to ELF-EMF increased both arachidonic acid (AA) and leukotriene E4 (LTE4) levels in HEK293 cells. CAY10502 and bestatin, which block the increase of AA and LTE4 respectively, abrogated the ELF-EMF inhibitory effect on Cav3.2 channels. Exogenous LTE4 mimicked the ELF-EMF inhibition of T-type calcium channels. ELF-EMF (50Hz) inhibits native T-type calcium channels in primary cultured mouse cortical neurons via LTE4. We conclude that 50Hz ELF-EMF inhibits T-type calcium channels through AA/LTE4 signaling pathway.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationNot supplied

Sample sizeNot supplied

ExposureELF · 0.05 MHz

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In HEK293 cells transfected with human T-type calcium channels, 50 Hz ELF-EMF exposure inhibited Cav3.1, Cav3.2, and Cav3.3 channel activity. ELF-EMF (50 Hz, 0.2 mT) did not alter Cav3.2 protein expression or steady-state activation/inactivation kinetics, but increased AA and LTE4 levels; blocking AA or LTE4 increases abrogated the inhibitory effect, and exogenous LTE4 mimicked it. ELF-EMF (50 Hz) also inhibited native T-type calcium channels in primary cultured mouse cortical neurons via LTE4.

Outcomes measured

T-type (LVA) calcium channel activity (Cav3.1, Cav3.2, Cav3.3)
Cav3.2 protein expression
Steady-state activation/inactivation kinetics of Cav3.2
Arachidonic acid (AA) levels
Leukotriene E4 (LTE4) levels
Native T-type calcium channel activity in primary cultured mouse cortical neurons

Limitations

In vitro/cell culture and primary neuron culture models; findings may not generalize to in vivo conditions
Exposure duration not reported in the abstract
Sample sizes not reported in the abstract
Mechanistic pathway inference based on pharmacologic blockers; potential off-target effects not described in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "T-type (LVA) calcium channel activity (Cav3.1, Cav3.2, Cav3.3)",
    "Cav3.2 protein expression",
    "Steady-state activation/inactivation kinetics of Cav3.2",
    "Arachidonic acid (AA) levels",
    "Leukotriene E4 (LTE4) levels",
    "Native T-type calcium channel activity in primary cultured mouse cortical neurons"
  ],
  "main_findings": "In HEK293 cells transfected with human T-type calcium channels, 50 Hz ELF-EMF exposure inhibited Cav3.1, Cav3.2, and Cav3.3 channel activity. ELF-EMF (50 Hz, 0.2 mT) did not alter Cav3.2 protein expression or steady-state activation/inactivation kinetics, but increased AA and LTE4 levels; blocking AA or LTE4 increases abrogated the inhibitory effect, and exogenous LTE4 mimicked it. ELF-EMF (50 Hz) also inhibited native T-type calcium channels in primary cultured mouse cortical neurons via LTE4.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro/cell culture and primary neuron culture models; findings may not generalize to in vivo conditions",
    "Exposure duration not reported in the abstract",
    "Sample sizes not reported in the abstract",
    "Mechanistic pathway inference based on pharmacologic blockers; potential off-target effects not described in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "0.2 mT",
    "T-type calcium channels",
    "Cav3.1",
    "Cav3.2",
    "Cav3.3",
    "HEK293",
    "mouse cortical neurons",
    "arachidonic acid",
    "leukotriene E4",
    "LTE4",
    "AA/LTE4 signaling pathway"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.ceca.2013.11.002
PubMed: 24360572

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