Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation

Ecotoxicology and environmental safety · 2021 · Mixed · Low evidence

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MixedLow evidencePaper 1072

Low-concentration DEHP (1 μM) promoted FL cell proliferation, while high-dose DEHP (100 μM) inhibited proliferation. Co-exposure to a 50-Hz, 0.2-mT magnetic field and 0.1 μM DEHP increased proliferation compared with single-factor exposure, with reported coop…

Study snapshot

PublicationEcotoxicology and environmental safety · 2021

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation Liangjing Chen, Anfang Ye, Xiaochen Liu, Jingchun Lu, Qixin Xie, Yixin Guo, Wenjun Sun. Combined effect of co- exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation. Ecotoxicol Environ Saf. 2021 Aug 26;224:112704. doi: 10.1016/j.ecoenv.2021.112704. Abstract Di (2-ethylhexyl) phthalate (DEHP) and extremely low-frequency electromagnetic fields (ELF-EMFs) exist far and wide in our surroundings. Studies have reported that both of DEHP and ELF-EMFs could promote cell proliferation which is related with adverse bioeffects. In this study, we investigated whether there is the combined effect between DEHP and 50-Hz magnetic fields (MFs) on cell proliferation in human amniotic (FL) cells. Results revealed that the low-concentration DEHP (1 μM) could promote FL cell proliferation, whereas the high-dose DEHP (100 μM) inhibited cell proliferation. When FL cells were treated jointly by a 50-Hz, 0.2-mT MF and 0.1 μM DEHP, the proliferation rate of cells was significantly higher than that of single factor exposure. Additionally, co-exposure to under-threshold MF and DEHP could cooperatively activate protein kinase B (Akt), sphingosine kinase 1 (SphK1) and extracellular signal regulated kinase (ERK) in a cascade manner, and finally mediate cell proliferation. Taken together, the findings of this study indicated that the co-exposure to under- threshold MF and DEHP could jointly promote cell proliferation through activating proliferation-related signal pathway, which warned us that it should be cautious about assessing the underlying health hazards of co- exposure to MFs and DEHP at under-threshold levels. Open access paper: pubmed.ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman amniotic (FL) cells

Sample sizeNot supplied

ExposureELF · 0.05 MHz

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Cell proliferation
Akt activation
SphK1 activation
ERK activation

Limitations

In vitro study in human amniotic (FL) cells; generalizability to humans/health outcomes not established in abstract
Exposure duration not reported in abstract
Sample size and replication details not reported in abstract
Co-exposure includes a chemical (DEHP), complicating attribution to magnetic-field exposure alone

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human amniotic (FL) cells",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation",
    "Akt activation",
    "SphK1 activation",
    "ERK activation"
  ],
  "main_findings": "Low-concentration DEHP (1 μM) promoted FL cell proliferation, while high-dose DEHP (100 μM) inhibited proliferation. Co-exposure to a 50-Hz, 0.2-mT magnetic field and 0.1 μM DEHP increased proliferation compared with single-factor exposure, with reported cooperative activation of Akt, SphK1, and ERK signaling.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study in human amniotic (FL) cells; generalizability to humans/health outcomes not established in abstract",
    "Exposure duration not reported in abstract",
    "Sample size and replication details not reported in abstract",
    "Co-exposure includes a chemical (DEHP), complicating attribution to magnetic-field exposure alone"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "DEHP",
    "di(2-ethylhexyl) phthalate",
    "ELF-EMF",
    "50-Hz magnetic field",
    "0.2 mT",
    "human amniotic cells",
    "FL cells",
    "cell proliferation",
    "Akt",
    "SphK1",
    "ERK",
    "co-exposure"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "Study involves ELF 50-Hz magnetic-field exposure, which can be relevant to occupational settings, though no specific setting is stated."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.ecoenv.2021.112704
PubMed: 34455183

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