Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields.

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2013 · Harm · Low evidence

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HarmLow evidencePaper 3086

Cultured HFSFs exposed to 0.2 mT ELF-EMF for 24 h showed increased MMP-2 mRNA (0.793-fold) and protein (1.130-fold) expression. Puerarin treatment (0.1–10 microM) reversed the ELF-EMF-associated increases, with greater reversal at higher concentrations (repor…

Study snapshot

PublicationJournal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2013

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

OBJECTIVE: To study the effect of puerarin on matrix metalloproteinase-2 (MMP-2) gene and protein expression in human fetal scleral fibroblasts (HFSFs) exposed to extremely low frequency electromagnetic fields (ELF-EMF). METHODS: Cultured HFSFs were exposed to 0.2 mT ELF-EMF for 24 h. The experimental groups were divided into subgroups treated with 0, 0.1, 1 and 10 microM puerarin respectively. The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively. RESULTS: MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for 24 h. Puerarin at the concentration of 0.1 microM reversed this effect by 8.53% in mRNA and by 17.97% in protein expression (P < 0.05). The effect was more prominent at higher concentrations (1 and 10 microM, P < 0.01). CONCLUSION: Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells. Puerarin reversed the action to some extent in a specific concentration range. Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationHuman fetal scleral fibroblasts (HFSFs) in culture

Sample sizeNot supplied

ExposureELF · 24 h

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

MMP-2 mRNA expression
MMP-2 protein expression

Limitations

In vitro cell culture model; findings may not translate to humans or tissues in vivo
Frequency of the ELF-EMF exposure not reported in the abstract
Sample size and replication details not reported in the abstract
Only one field intensity (0.2 mT) and one exposure duration (24 h) reported

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "24 h"
  },
  "population": "Human fetal scleral fibroblasts (HFSFs) in culture",
  "sample_size": null,
  "outcomes": [
    "MMP-2 mRNA expression",
    "MMP-2 protein expression"
  ],
  "main_findings": "Cultured HFSFs exposed to 0.2 mT ELF-EMF for 24 h showed increased MMP-2 mRNA (0.793-fold) and protein (1.130-fold) expression. Puerarin treatment (0.1–10 microM) reversed the ELF-EMF-associated increases, with greater reversal at higher concentrations (reported as statistically significant).",
  "effect_direction": "harm",
  "limitations": [
    "In vitro cell culture model; findings may not translate to humans or tissues in vivo",
    "Frequency of the ELF-EMF exposure not reported in the abstract",
    "Sample size and replication details not reported in the abstract",
    "Only one field intensity (0.2 mT) and one exposure duration (24 h) reported"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency electromagnetic fields",
    "ELF-EMF",
    "0.2 mT",
    "human fetal scleral fibroblasts",
    "HFSFs",
    "matrix metalloproteinase-2",
    "MMP-2",
    "puerarin",
    "gene expression",
    "protein expression",
    "RT-PCR",
    "western blot"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/s0254-6272(14)60039-x
PubMed: 24660593

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