The effect of extremely low frequency pulsed electromagnetic field on in vitro fertilization success rate in N MRI mice.

Cell journal · 2014 · No effect · Very low evidence

← Research stream

No effectVery low evidencePaper 4554

IVF success rate was 77% in the ELF pulsed electromagnetic field exposed group versus 68% in controls, but the difference was not statistically significant.

Study snapshot

PublicationCell journal · 2014

Study typeanimal

Reported directionNo effect

Evidence ratingVery low

Abstract

OBJECTIVE: The effects of exposure to electromagnetic fields (EMF) on reproduction systems have been widely debated. In this study, we aimed to investigate whether low frequency EMF could ameliorate the in vitro fertilization success rate in Naval medical research institute (NMRI) Mice. MATERIALS AND METHODS: In this randomized comparative animal study, ten NMRI mice were randomly divided into 2 equal groups (control and experimental). 10 IU of human chorionic gonadotropin (hCG) was injected intraperitoneally to both groups in order to stimulate ovulating, and ovums were then aspirated and kept in KSOM (modified version of sequential simplex optimization medium with a higher K(+) concentration) culture medium. Metaphase II ovums were separated, and sperms obtained by "swim out" method were added to metaphase II ovums in the culture medium. The experimental group was exposed to 1.3 millitesla pulsed electromagnetic field at 4 kilohertz frequency for 5 hours. To assess the efficacy, we considered the identification of two-pronuclear zygote (2PN) under microscope as fertilizing criterion. RESULTS: Total number of collected ovums in the control and experimental groups was 191 and 173, respectively, from which 58 (30.05%) and 52 (30.36%) ovums were collected from metaphase II, respectively. In vitro fertilization (IVF) success rate was 77% in extremely low frequency- pulsed electromagnetic field (ELFPEMF) for exposed group (experimental), whereas the rate was 68% for control group. CONCLUSION: Despite increased percentile of IVF success rate in exposed group, there was no statistically significant difference between 2 groups, but this hypothesis has still been stated as a question. Further studies with larger sample sizes and different EMF designs are suggested.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionno_effect

PopulationNaval medical research institute (NMRI) mice

Sample size10

ExposureELF · 0.004 MHz · 5 hours

Evidence strengthVery lowConfidence: 78% · Peer reviewed: yes

Main findings

IVF success rate was 77% in the ELF pulsed electromagnetic field exposed group versus 68% in controls, but the difference was not statistically significant.

Outcomes measured

In vitro fertilization (IVF) success rate (2-pronuclear zygote, 2PN)
Number of collected ovums
Number/percent of metaphase II ovums

Limitations

Small sample size (10 mice)
No statistically significant difference reported despite higher IVF percentage in exposed group
Authors indicate need for larger studies and different EMF designs

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.004,
    "sar_wkg": null,
    "duration": "5 hours"
  },
  "population": "Naval medical research institute (NMRI) mice",
  "sample_size": 10,
  "outcomes": [
    "In vitro fertilization (IVF) success rate (2-pronuclear zygote, 2PN)",
    "Number of collected ovums",
    "Number/percent of metaphase II ovums"
  ],
  "main_findings": "IVF success rate was 77% in the ELF pulsed electromagnetic field exposed group versus 68% in controls, but the difference was not statistically significant.",
  "effect_direction": "no_effect",
  "limitations": [
    "Small sample size (10 mice)",
    "No statistically significant difference reported despite higher IVF percentage in exposed group",
    "Authors indicate need for larger studies and different EMF designs"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "pulsed electromagnetic field",
    "ELFPEMF",
    "in vitro fertilization",
    "reproduction",
    "NMRI mice",
    "4 kHz",
    "1.3 mT",
    "2PN zygote"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 24381855

Read the original paper →

Related papers

← Back to the Research Library