Electromagnetic fields with frequencies of 5, 60 and 120 Hz affect the cell cycle and viability of human fibroblast BJ in vitro

2017 · Mixed · Low evidence

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MixedLow evidencePaper 2335

After 2 hours exposure at 2.5 mT, pulsed EMFs at 5, 60, and 120 Hz decreased BJ cell viability, and reduced viability persisted 24 hours post-exposure. Continuous EMF reduced viability only at 120 Hz and the reduction persisted 24 hours; continuous 5 Hz showe…

Study snapshot

Publicationmanual · 2017

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Electromagnetic fields with frequencies of 5, 60 and 120 Hz affect the cell cycle and viability of human fibroblast BJ in vitro Koziorowska A, Romerowicz-Misielak M Filipek A, Koziorowski M. Electromagnetic fields with frequencies of 5, 60 and 120 Hz affect the cell cycle and viability of human fibroblast BJ in vitro. J Biol Regul Homeost Agents. 2017 Jul-Sep;31(3):725-730. Abstract The impact of electromagnetic field (EMF) on humans has been described in numerous studies, but many questions are still unanswered. The aim of the experiment described in this study was to evaluate the effect of EMF on the viability of human fibroblast BJ in vitro and the percentage of cells in different phases of the cell cycle (G1/G0, S, G2/M) after 2 hours of exposure to sinusoidal continuous and pulsed EMFs with frequency of 5 Hz, 60 Hz and 120 Hz at a magnetic induction of 2,5 mT. The viability of BJ cells exposed to an EMF was estimated immediately after completion of exposure and after 24 hours. Metabolic activity of cells was assessed by MTT assay and compared to a control culture not exposed to EMFs. Cell cycle analysis was performed by BrdU incorporation. The analysis of the viability demonstrated significant differences in field efficiency, depending on its nature. Exposure of cells to pulse EMFs resulted in a decrease in their viability for each of the analyzed frequencies. Reduced viability was maintained for a further 24 hours after the end of exposure of cells to pulsed EMF. In the case of continuous field, reduced BJ cell viability was observed only at the highest applied frequency - 120Hz, and this effect maintained for the next 24 hours. Although there was no significant effect on cell viability (metabolic activity) of cells immediately after exposure to continuous EMF with a frequency of 5Hz, a significant increase was observed after 24 hours of incubation. ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman fibroblast BJ cells (in vitro)

Sample sizeNot supplied

ExposureELF · 2 hours

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Cell viability/metabolic activity (MTT assay)
Cell cycle phase distribution (G1/G0, S, G2/M) via BrdU incorporation

Limitations

Sample size not reported in abstract
Details of exposure setup beyond frequency, waveform (continuous vs pulsed), duration, and magnetic induction are not provided
Results for cell cycle phase distribution are not described in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "2 hours"
  },
  "population": "Human fibroblast BJ cells (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Cell viability/metabolic activity (MTT assay)",
    "Cell cycle phase distribution (G1/G0, S, G2/M) via BrdU incorporation"
  ],
  "main_findings": "After 2 hours exposure at 2.5 mT, pulsed EMFs at 5, 60, and 120 Hz decreased BJ cell viability, and reduced viability persisted 24 hours post-exposure. Continuous EMF reduced viability only at 120 Hz and the reduction persisted 24 hours; continuous 5 Hz showed no immediate viability effect but a significant increase after 24 hours.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Details of exposure setup beyond frequency, waveform (continuous vs pulsed), duration, and magnetic induction are not provided",
    "Results for cell cycle phase distribution are not described in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF EMF",
    "5 Hz",
    "60 Hz",
    "120 Hz",
    "pulsed electromagnetic field",
    "continuous electromagnetic field",
    "2.5 mT",
    "human fibroblast BJ",
    "MTT assay",
    "BrdU incorporation",
    "cell cycle",
    "cell viability"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "ELF magnetic field frequencies (5–120 Hz) are relevant to some occupational/industrial exposures, though the study is in vitro and no specific workplace source is stated."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: Not supplied

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