Intermittent Exposure to a 16 Hz Extremely Low Frequency Pulsed Electromagnetic Field Promotes Osteogenesis In Vitro through Activating Piezo 1-Induced Ca Influx in Osteoprogenitor Cells.

Journal of functional biomaterials · 2023 · Benefit · Low evidence

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BenefitLow evidencePaper 5803

Study snapshot

PublicationJournal of functional biomaterials · 2023

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

Exposure to extremely low frequency pulsed electromagnetic fields (ELF-PEMF) is supposed to simulate local EMF generated during mechanical stimulation of bone and may therefore be used to improve bone regeneration. This study aimed at optimizing the exposure strategy and investigating the underlying mechanisms of a 16 Hz ELF-PEMF, previously reported to boost osteoblast function. Comparing influences of daily continuous (30 min every 24 h) and intermittent (10 min every 8 h) exposure to the 16 Hz ELF-PEMF on osteoprogenitor cells revealed that the intermittent exposure strategy enhanced the 16 Hz ELF-PEMF effects regarding cell numbers and osteogenic function. Gene expression of and related Ca influx were significantly increased in SCP-1 cells with the daily intermittent exposure. Pharmacological inhibition of piezo 1 with Dooku 1 largely abolished the positive effect of the 16 Hz ELF-PEMF exposure on osteogenic maturation of SCP-1 cells. In summary, the intermittent exposure strategy enhanced the positive effects of 16 Hz continuous ELF-PEMF exposure in terms of cell viability and osteogenesis. This effect was shown to be mediated by an increased expression of and related Ca influx. Thus, the intermittent exposure strategy is a promising way to further optimize the therapeutic effects of the 16 Hz ELF-PEMF regarding fracture healing or osteoporosis.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationOsteoprogenitor cells (SCP-1 cells)

Sample sizeNot supplied

ExposureELF · continuous: 30 min every 24 h; intermittent: 10 min every 8 h (daily)

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In osteoprogenitor (SCP-1) cells, daily intermittent exposure (10 min every 8 h) to a 16 Hz ELF-PEMF enhanced effects on cell numbers and osteogenic function compared with daily continuous exposure (30 min every 24 h). Piezo1 expression and related Ca2+ influx increased with intermittent exposure, and pharmacological inhibition of Piezo1 (Dooku 1) largely abolished the positive effects on osteogenic maturation.

Outcomes measured

cell numbers
cell viability
osteogenic function/osteogenesis
osteogenic maturation
Piezo1 gene expression
Ca2+ influx

Limitations

In vitro study; findings may not translate to clinical outcomes (e.g., fracture healing or osteoporosis).
No sample size reported in the provided abstract.
Exposure intensity/dose metrics beyond frequency and timing not reported in the provided abstract.

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "continuous: 30 min every 24 h; intermittent: 10 min every 8 h (daily)"
  },
  "population": "Osteoprogenitor cells (SCP-1 cells)",
  "sample_size": null,
  "outcomes": [
    "cell numbers",
    "cell viability",
    "osteogenic function/osteogenesis",
    "osteogenic maturation",
    "Piezo1 gene expression",
    "Ca2+ influx"
  ],
  "main_findings": "In osteoprogenitor (SCP-1) cells, daily intermittent exposure (10 min every 8 h) to a 16 Hz ELF-PEMF enhanced effects on cell numbers and osteogenic function compared with daily continuous exposure (30 min every 24 h). Piezo1 expression and related Ca2+ influx increased with intermittent exposure, and pharmacological inhibition of Piezo1 (Dooku 1) largely abolished the positive effects on osteogenic maturation.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study; findings may not translate to clinical outcomes (e.g., fracture healing or osteoporosis).",
    "No sample size reported in the provided abstract.",
    "Exposure intensity/dose metrics beyond frequency and timing not reported in the provided abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF-PEMF",
    "pulsed electromagnetic field",
    "16 Hz",
    "intermittent exposure",
    "osteogenesis",
    "osteoprogenitor cells",
    "SCP-1",
    "Piezo1",
    "calcium influx",
    "Dooku 1"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3390/jfb14030165
PubMed: 36976089

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