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Effects of 50 Hz electromagnetic fields on human epidermal stem cells cultured on collagen sponge scaffolds.

International journal of radiation biology · 2012 · Benefit · Low evidence

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BenefitLow evidencePaper 4530

Human epidermal stem cells cultured in collagen sponge scaffolds grew steadily, and exposure to a 50 Hz, 5 mT electromagnetic field for 14 days (30 min/day) promoted ESC proliferation compared with control (P < 0.05).

Study snapshot

PublicationInternational journal of radiation biology · 2012
Study typein_vitro
Reported directionBenefit
Evidence ratingLow

Abstract

PURPOSE: This study is to investigate the effects of electromagnetic fields (EMF) on proliferation of epidermal stem cells (ESC), which could present a viable clinical option for skin tissue engineering. MATERIALS AND METHODS: The ESC obtained from human foreskin were grafted into type-I three-dimensional collagen sponge scaffolds, and then were exposed with EMF (frequency 50 Hz, intensity 5 mT) for 14 d (30 min per d). Meanwhile, the control group was set under the same conditions without EMF. The effects of EMF on growth and proliferation of ESC were analyzed with staining of hematoxylin and eosin (H&E) and 4',6-diamidino-2-phenylindole (DAPI) under microscope or scanning electron microscope. The data of DAPI staining for 2 d, 7 d, 10 d and 14 d were collected respectively to investigate the cells proliferation. RESULTS: ESC cultured in collagen sponge scaffolds could be steady grown and EMF could promote ESC proliferation compared with control (P < 0.05). CONCLUSIONS: EMF could significantly promote proliferation of ESC, which leads to a promising clinical option for skin tissue engineering.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationHuman epidermal stem cells obtained from human foreskin, cultured on type-I 3D collagen sponge scaffolds
Sample sizeNot supplied
ExposureELF · 0.05 MHz · 14 d (30 min per d)
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Human epidermal stem cells cultured in collagen sponge scaffolds grew steadily, and exposure to a 50 Hz, 5 mT electromagnetic field for 14 days (30 min/day) promoted ESC proliferation compared with control (P < 0.05).

Outcomes measured

  • Epidermal stem cell (ESC) growth in collagen sponge scaffolds
  • ESC proliferation (DAPI staining cell counts at 2, 7, 10, 14 days; microscopy/SEM observations)

Limitations

  • Sample size not reported in abstract
  • In vitro cell culture model; clinical relevance not established in abstract
  • Exposure parameters limited to a single frequency (50 Hz) and intensity (5 mT)
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "14 d (30 min per d)"
  },
  "population": "Human epidermal stem cells obtained from human foreskin, cultured on type-I 3D collagen sponge scaffolds",
  "sample_size": null,
  "outcomes": [
    "Epidermal stem cell (ESC) growth in collagen sponge scaffolds",
    "ESC proliferation (DAPI staining cell counts at 2, 7, 10, 14 days; microscopy/SEM observations)"
  ],
  "main_findings": "Human epidermal stem cells cultured in collagen sponge scaffolds grew steadily, and exposure to a 50 Hz, 5 mT electromagnetic field for 14 days (30 min/day) promoted ESC proliferation compared with control (P < 0.05).",
  "effect_direction": "benefit",
  "limitations": [
    "Sample size not reported in abstract",
    "In vitro cell culture model; clinical relevance not established in abstract",
    "Exposure parameters limited to a single frequency (50 Hz) and intensity (5 mT)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "ELF electromagnetic fields",
    "5 mT",
    "epidermal stem cells",
    "human foreskin",
    "collagen sponge scaffold",
    "tissue engineering",
    "cell proliferation",
    "DAPI",
    "H&E",
    "scanning electron microscopy"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3109/09553002.2012.692496
PubMed: 22568519

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