P-selectin expression in a colon tumor model exposed by sinusoidal electromagnetic fields.

Biomedical reports · 2013 · No effect · Low evidence

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No effectLow evidencePaper 3089

Rats exposed to a 50 Hz, 5 mT sinusoidal magnetic field (with or without MNU treatment) showed no statistically significant change (P>0.05) in P-selectin expression in colon tissues compared with groups not exposed to the field.

Study snapshot

PublicationBiomedical reports · 2013

Study typeanimal

Reported directionNo effect

Evidence ratingLow

Abstract

P-selectin is mainly involved in the initial process of tumor cell adhesion to platelets. The aim of the present study was to determine the expression level of P-selectin in a colon tumor model affected by sinusoidal electromagnetic fields (SMF). Male Wistar albino rats aged 2-2.5 months were used. The animals were divided into the I [N-Methyl-N-Nitrosurea (MNU)], II (SMF-MNU), III (SMF) and IV (control) groups. The rats were housed five per polycarbonate cage. Sixty milligrams of MNU was dissolved in 6 ml sterile 0.9% NaCl. Prepared solutions were administered intra rectally (i.r.) to the 1st and 3rd groups as 0.2 ml/per animal. The same procedure was applied to the 2nd and 4th groups, although 0.2 ml/per animal sterile isotonic solution was administered instead. This procedure was repeated once a week for 10 weeks. Following the administration of MNU, the 2nd and 3rd groups were exposed to a sinusoidal magnetic field (SMF, 50 Hz, 5 mT) for 6 h/day for 8 months. P-selectin expression of the four groups of rat colon tissues was determined using immunohistochemistry on paraffin sections. The labeled streptavidin biotin method was performed. Fisher's exact test was used for differences between proportions. Results showed that there was no statistically significant (P>0.05) change in the expression level of P-selectin. However, this result should be verified by both and experiments to determine the effects of the magnetic fields on P-selectin.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionno_effect

PopulationMale Wistar albino rats (2–2.5 months old) in a colon tumor model (MNU-induced)

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 6 h/day for 8 months

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

P-selectin expression in rat colon tissue (immunohistochemistry)

Limitations

Sample size not reported in the abstract
Only P-selectin expression assessed; other tumor or biological endpoints not described
Authors note findings should be verified by additional experiments (types not specified in abstract)

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": "sinusoidal magnetic field",
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "6 h/day for 8 months"
  },
  "population": "Male Wistar albino rats (2–2.5 months old) in a colon tumor model (MNU-induced)",
  "sample_size": null,
  "outcomes": [
    "P-selectin expression in rat colon tissue (immunohistochemistry)"
  ],
  "main_findings": "Rats exposed to a 50 Hz, 5 mT sinusoidal magnetic field (with or without MNU treatment) showed no statistically significant change (P>0.05) in P-selectin expression in colon tissues compared with groups not exposed to the field.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in the abstract",
    "Only P-selectin expression assessed; other tumor or biological endpoints not described",
    "Authors note findings should be verified by additional experiments (types not specified in abstract)"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "P-selectin",
    "colon tumor model",
    "N-Methyl-N-Nitrosurea",
    "sinusoidal magnetic field",
    "ELF magnetic field",
    "50 Hz",
    "5 mT",
    "immunohistochemistry",
    "rat"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "ELF magnetic-field exposure parameters (50 Hz) may be relevant to occupational/ELF exposure discussions, though this is an animal model."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3892/br.2013.81
PubMed: 24648955

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