Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model.

Bioelectromagnetics · 2017 · Benefit · Low evidence

← Research stream

BenefitLow evidencePaper 4934

In a murine full-thickness wound model with human ESC transplantation, the ESC+EMF group (50 Hz, 5 mT, 60 min/day for 20 days) had faster wound healing than the other groups (P<0.05). Regenerated skin in the ESC+EMF group was described as more mature with mor…

Study snapshot

PublicationBioelectromagnetics · 2017

Study typeanimal

Reported directionBenefit

Evidence ratingLow

Abstract

To explore the possible efficacy of electromagnetic fields (EMF) for skin tissue engineering, effects of EMF exposure on epidermal stem cells (ESC) seeded in collagen sponge scaffolds for wound healing in a murine model were investigated. The wound models of a full-thickness defect established with 36 7 ∼ 8-week-old nude mice were randomly divided into three groups: a control group, an ESC-only group, and an ESC with EMF exposure group (frequency of 50 Hz, magnetic induction of 5 mT, 60 min per day for 20 days). ESC were separated from human foreskin and cultured in vitro, and then transplanted with collagen sponge scaffolds as a delivery vehicle to wounds of the ESC-only group, and ESC with EMF exposure group was exposed to EMF after ESC transplantation. Effects of EMF on morphological changes and expression of β1 integrin in regenerated skins were observed. Wound healing rates and healing times were collected to evaluate the efficacy of repairment. Results showed that human ESC were successfully transplanted to nude mice, which facilitated the formation of intact skin on nude mice. In contrast to other groups, the wound healing of ESC with EMF exposure group was the fastest (P < 0.05), the structure of regenerated skins was more mature, and it contained more continuity in the number of viable cell layers and rich hair follicles' structure. These results suggest that the use of 50 Hz EMF as a non-invasive treatment can accelerate wound healing of ESC transplantation, and restore structural integrity of regenerated skin. Bioelectromagnetics. 38:204-212,2017. © 2017 Wiley Periodicals, Inc.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionbenefit

PopulationNude mice with full-thickness skin wounds receiving human epidermal stem cell (ESC) transplantation in collagen sponge scaffolds

Sample size36

ExposureELF · 0.05 MHz · 60 min/day for 20 days

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Wound healing rate
Healing time
Morphological changes in regenerated skin
β1 integrin expression in regenerated skin
Maturity/structure of regenerated skin (viable cell layers, hair follicle structures)

Limitations

Murine model (nude mice) may not generalize to humans
Human ESC were used but outcomes were assessed in mice
Only one EMF exposure condition reported (50 Hz, 5 mT; 60 min/day for 20 days)
Abstract does not report quantitative effect sizes for healing outcomes
Blinding and allocation concealment not described in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "60 min/day for 20 days"
  },
  "population": "Nude mice with full-thickness skin wounds receiving human epidermal stem cell (ESC) transplantation in collagen sponge scaffolds",
  "sample_size": 36,
  "outcomes": [
    "Wound healing rate",
    "Healing time",
    "Morphological changes in regenerated skin",
    "β1 integrin expression in regenerated skin",
    "Maturity/structure of regenerated skin (viable cell layers, hair follicle structures)"
  ],
  "main_findings": "In a murine full-thickness wound model with human ESC transplantation, the ESC+EMF group (50 Hz, 5 mT, 60 min/day for 20 days) had faster wound healing than the other groups (P<0.05). Regenerated skin in the ESC+EMF group was described as more mature with more continuous viable cell layers and richer hair follicle structures.",
  "effect_direction": "benefit",
  "limitations": [
    "Murine model (nude mice) may not generalize to humans",
    "Human ESC were used but outcomes were assessed in mice",
    "Only one EMF exposure condition reported (50 Hz, 5 mT; 60 min/day for 20 days)",
    "Abstract does not report quantitative effect sizes for healing outcomes",
    "Blinding and allocation concealment not described in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "extremely low frequency",
    "electromagnetic fields",
    "magnetic induction",
    "5 mT",
    "wound healing",
    "skin tissue engineering",
    "epidermal stem cells",
    "collagen sponge scaffold",
    "murine model",
    "β1 integrin"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.22029
PubMed: 28106913

Read the original paper →

Related papers

← Back to the Research Library