Expression pattern of the Ets-related transcription factor Elf-1.

Molecular medicine (Cambridge, Mass.) · 1998 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 4057

Study snapshot

PublicationMolecular medicine (Cambridge, Mass.) · 1998

Study typeother

Reported directionUnclear

Evidence ratingInsufficient

Abstract

Members of the Ets family of winged helix-loop-helix transcription factors play important roles in the development and function of multiple mammalian cell lineages. Elf-1 is an Ets-related transcription factor that is expressed at high levels in T cells and is known to regulate the expression of several T cell genes, including the granulocyte-macrophage colony stimulating factor (GM-CSF) gene, the interleukin-2 receptor alpha subunit (IL-2Ralpha) gene, and the CD4 gene. In the studies described in this report, we have characterized the pattern of expression of Elf-1 in the developing mouse embryo and in adult mouse tissues as well as in multiple immortalized human and murine cell lines. Elf-1 is expressed at high levels throughout thymocyte development, with equivalent levels of Elf-1 expression seen in all subsets of maturing thymocytes and T cells. Somewhat surprisingly, however, Elf-1 is also expressed at high levels in epithelial cells lining the oral cavity, the lung, the CNS, and the gastrointestinal and urinary tracts as well as in the skin of the developing mouse embryo and at lower levels in the adult mouse testis and liver. Western blot analyses of a large number of immortalized cell lines demonstrated high-level Elf-1 expression in T and B lymphocyte and macrophage cell lines as well as in two prostate carcinoma cell lines. Low-level expression was observed in fibroblasts, embryonic stem cells, and myoblasts. Taken together, our data suggest that in addition to its role in regulating T cell development and function, Elf-1 may regulate gene expression in the B cell and myelomonocytic lineages, as well as in multiple epithelial cell types during murine embryonic development.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother

Effect directionunclear

PopulationDeveloping mouse embryo and adult mouse tissues; multiple immortalized human and murine cell lines

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthInsufficientConfidence: 86% · Peer reviewed: yes

Main findings

Elf-1 was reported to be expressed at high levels throughout thymocyte development with similar expression across maturing thymocyte and T cell subsets. High-level expression was also observed in several embryonic epithelial tissues, with lower levels in adult mouse testis and liver. Western blot analyses indicated high Elf-1 expression in T and B lymphocyte and macrophage cell lines and in two prostate carcinoma cell lines, with low-level expression in fibroblasts, embryonic stem cells, and myoblasts.

Outcomes measured

Elf-1 expression pattern across thymocyte development and T cell subsets
Elf-1 expression in embryonic epithelial tissues (oral cavity, lung, CNS, gastrointestinal and urinary tracts, skin)
Elf-1 expression in adult mouse tissues (testis, liver)
Elf-1 protein expression in immortalized cell lines (immune lineages, prostate carcinoma, fibroblasts, embryonic stem cells, myoblasts)

Limitations

No EMF exposure or comparison group described in the provided abstract
Sample sizes and quantitative effect estimates are not reported
Methods for measuring expression are only briefly mentioned (e.g., Western blot) without detailed protocol in the abstract

View raw extracted JSON

{
  "study_type": "other",
  "exposure": {
    "band": null,
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Developing mouse embryo and adult mouse tissues; multiple immortalized human and murine cell lines",
  "sample_size": null,
  "outcomes": [
    "Elf-1 expression pattern across thymocyte development and T cell subsets",
    "Elf-1 expression in embryonic epithelial tissues (oral cavity, lung, CNS, gastrointestinal and urinary tracts, skin)",
    "Elf-1 expression in adult mouse tissues (testis, liver)",
    "Elf-1 protein expression in immortalized cell lines (immune lineages, prostate carcinoma, fibroblasts, embryonic stem cells, myoblasts)"
  ],
  "main_findings": "Elf-1 was reported to be expressed at high levels throughout thymocyte development with similar expression across maturing thymocyte and T cell subsets. High-level expression was also observed in several embryonic epithelial tissues, with lower levels in adult mouse testis and liver. Western blot analyses indicated high Elf-1 expression in T and B lymphocyte and macrophage cell lines and in two prostate carcinoma cell lines, with low-level expression in fibroblasts, embryonic stem cells, and myoblasts.",
  "effect_direction": "unclear",
  "limitations": [
    "No EMF exposure or comparison group described in the provided abstract",
    "Sample sizes and quantitative effect estimates are not reported",
    "Methods for measuring expression are only briefly mentioned (e.g., Western blot) without detailed protocol in the abstract"
  ],
  "evidence_strength": "insufficient",
  "confidence": 0.86,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "Elf-1",
    "Ets family",
    "transcription factor",
    "gene expression",
    "thymocyte development",
    "T cells",
    "mouse embryo",
    "epithelial cells",
    "Western blot",
    "cell lines"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 10780882

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