Binding sites for transcription factor NTF-1/Elf-1 contribute to the ventral repression of decapentaplegic.

Genes & development · 1995 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 4280

Study snapshot

PublicationGenes & development · 1995

Study typeother

Reported directionUnclear

Evidence ratingInsufficient

Abstract

The Dorsal morphogen is a transcription factor that activates some genes and represses others to establish multiple domains of gene expression along the dorsal/ventral axis of the early Drosophila embryo. Repression by Dorsal appears to require accessory proteins that bind to corepression elements in Dorsal-dependent regulatory modules called ventral repression regions (VRRs). We have identified a corepression element in decapentaplegic (dpp), a zygotically active gene that is repressed by the Dorsal morphogen. This dpp repression element (DRE) is located within a previously identified VRR and close to essential Dorsal-binding sites. We have purified a factor from Drosophila embryo extracts that binds to the DRE but not to mutant forms of the DRE that fail to support efficient repression. This protein also binds to an apparently essential region in a VRR associated with the zerknüllt (zen) gene. One of the DREs in the dpp VRR overlaps the binding site for a potential activator protein suggesting that one mechanism of ventral repression may be the mutually exclusive binding of repressor and activator proteins. We have found the DRE-binding protein to be identical to NTF-1 (equivalent to Elf-1, the product of the grainyhead gene), a factor originally identified as an activator of the Ultrabithorax and Dopa decarboxylase promoters. NTF-1 mRNA is synthesized during oogenesis and deposited in the developing oocyte where it is available to contribute to ventral repression during early embryogenesis. Previous studies have shown that overexpression of NTF-1 in the postblastoderm embryo results in a phenotype that is consistent with a role for this factor in the repression of dpp later in embryogenesis.

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AI evidence extraction

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Study typeother

Effect directionunclear

PopulationNot supplied

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthInsufficientConfidence: 74% · Peer reviewed: yes

Main findings

A corepression element (DRE) within the dpp ventral repression region was identified, and a protein from Drosophila embryo extracts was purified that binds the DRE but not mutant DREs that fail to support efficient repression. The DRE-binding protein was identified as NTF-1 (Elf-1; grainyhead gene product), and the abstract notes NTF-1 mRNA is deposited during oogenesis, consistent with a role in early embryonic ventral repression.

Outcomes measured

Identification of a decapentaplegic (dpp) repression element (DRE) within a ventral repression region (VRR)
Purification/identification of a DRE-binding protein from Drosophila embryo extracts
Evidence that the DRE-binding protein is NTF-1/Elf-1 (grainyhead gene product)
Description of NTF-1 mRNA deposition during oogenesis and potential role in ventral repression

View raw extracted JSON

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  "study_type": "other",
  "exposure": {
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  "outcomes": [
    "Identification of a decapentaplegic (dpp) repression element (DRE) within a ventral repression region (VRR)",
    "Purification/identification of a DRE-binding protein from Drosophila embryo extracts",
    "Evidence that the DRE-binding protein is NTF-1/Elf-1 (grainyhead gene product)",
    "Description of NTF-1 mRNA deposition during oogenesis and potential role in ventral repression"
  ],
  "main_findings": "A corepression element (DRE) within the dpp ventral repression region was identified, and a protein from Drosophila embryo extracts was purified that binds the DRE but not mutant DREs that fail to support efficient repression. The DRE-binding protein was identified as NTF-1 (Elf-1; grainyhead gene product), and the abstract notes NTF-1 mRNA is deposited during oogenesis, consistent with a role in early embryonic ventral repression.",
  "effect_direction": "unclear",
  "limitations": [],
  "evidence_strength": "insufficient",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "Dorsal morphogen",
    "transcriptional repression",
    "ventral repression region (VRR)",
    "decapentaplegic (dpp)",
    "dpp repression element (DRE)",
    "zerknüllt (zen)",
    "NTF-1",
    "Elf-1",
    "grainyhead",
    "Drosophila embryo",
    "corepression element"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1101/gad.9.24.3177
PubMed: 8543160

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