Splice isoforms of transcription factor Elf-1 affecting its regulatory function in transcription-molecular cloning of rat Elf-1.

Bioscience, biotechnology, and biochemistry · 2000 · Unclear · Insufficient evidence

← Research stream

UnclearInsufficient evidencePaper 3978

Rat Elf-1 cDNAs were cloned and two splice isoforms were identified in addition to full-length Elf-1. In a transient coexpression system, overexpression of rat Elf-1 type 1 inhibited alpha chain promoter activity; type 2 showed lower inhibitory activity than …

Study snapshot

PublicationBioscience, biotechnology, and biochemistry · 2000

Study typein_vitro

Reported directionUnclear

Evidence ratingInsufficient

Abstract

To elucidate the role of Elf-1 in Fc epsilonRI alpha chain expression, rat Elf-1 cDNAs were isolated and characterized. The rat Elf-1 cDNA of 2744 bp contained an open reading frame of 1848 bp. In addition to the full length rat Elf-1 cDNA (named type 1), two splice isoforms were isolated. One of the two isoforms lacked the amino acid residues from 85th to 120th (type 2), and the other from 85th to 175th (type 3). Similar isoforms were also observed in human tissue. Overexpression of rat Elf-1 (type 1) using a transient coexpression system inhibited of the alpha chain promoter activity. The inhibition activity was different between the isoforms; the inhibition activity of type 2 was lower than that of type 1, and type 3 did not have an inhibitory effect. This observation suggested that each Elf-1 isoform played a different role in the gene expression under its control.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionunclear

PopulationNot supplied

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthInsufficientConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Elf-1 splice isoforms (type 1, type 2, type 3) characterization
Fc epsilonRI alpha chain promoter activity (alpha chain promoter inhibition)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Elf-1 splice isoforms (type 1, type 2, type 3) characterization",
    "Fc epsilonRI alpha chain promoter activity (alpha chain promoter inhibition)"
  ],
  "main_findings": "Rat Elf-1 cDNAs were cloned and two splice isoforms were identified in addition to full-length Elf-1. In a transient coexpression system, overexpression of rat Elf-1 type 1 inhibited alpha chain promoter activity; type 2 showed lower inhibitory activity than type 1, and type 3 showed no inhibitory effect.",
  "effect_direction": "unclear",
  "limitations": [],
  "evidence_strength": "insufficient",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "Elf-1",
    "splice isoforms",
    "rat",
    "human tissue",
    "molecular cloning",
    "transcription factor",
    "Fc epsilonRI alpha chain",
    "promoter activity",
    "transient coexpression"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1271/bbb.64.2601
PubMed: 11210123

Read the original paper →

Related papers

← Back to the Research Library