Morphometric evaluation of Japanese quail embryos and their extraembryonic vascular networks exposed to low-frequency magnetic field with two different intensities

Electromagnetic biology and medicine · 2020 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 2249

Japanese quail embryos were exposed to a 60 Hz magnetic field at 0.16 mT or 0.65 mT (6 h). Fractal dimension measures of the yolk sac membrane vascular network and embryonic body mass did not differ among groups, but embryos exposed to 0.65 mT showed reduced …

Study snapshot

PublicationElectromagnetic biology and medicine · 2020

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

Morphometric evaluation of Japanese quail embryos and their extraembryonic vascular networks exposed to low-frequency magnetic field with two different intensities Edbhergue Ventura Lola Costa, Victor Felipe da Silva Araújo, Ana Paula Pereira Santos, Romildo de Albuquerque Nogueira. Morphometric evaluation of Japanese quail embryos and their extraembryonic vascular networks exposed to low-frequency magnetic field with two different intensities. Electromagn Biol Med. 2020 Sep 19;1-8. doi: 10.1080/15368378.2020.1821708. Abstract Animals developed or in an embryonic stage, are constantly subjected to magnetic pollution generated by electrical and electronic devices. Several researches have used the bird embryo as an experimental model to evaluate the action of magnetic field (MF) and electromagnetic field (EMF). This study proposed to perform a morphometric evaluation in the embryos and in the blood vascular network of the yolk sac membranes (YSM) of Japanese quail (Coturnix japonica) exposed to the 60 Hz MF with two different intensities (0.16 and 0.65 mT). A total of 30 eggs were used, 10 eggs were used for each assay. Each assay formed a group (control group, group submitted to the MF of 0.16 mT and 0.65 mT). The images of the skeletonized vascular network of YSM were evaluated by two methods of fractal dimension: box-counting dimension (Dbc) and information dimension (Dinf). The embryos were evaluated by body mass, percentage cephalic length and body area. The fractal dimensions revealed no difference among groups. There were no significant differences in relation to embryonic body mass among groups. However, the embryos exposed to 0.65 mT MF presented a smaller embryonic body development (body area and percentage cephalic length). In conclusion, 0.16 mT and 0.65 mT magnetic fields were not able to generate significant effects on vasculogenesis and angiogenesis. However, the embryos exposed to 6 h of magnetic field with 0.65 mT intensity and 60 Hz frequency showed a decrease in embryonic body development. pubmed.ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionmixed

PopulationJapanese quail (Coturnix japonica) embryos/eggs

Sample size30

ExposureELF · 6e-05 MHz · 6 h

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Yolk sac membrane (YSM) vascular network fractal dimensions (box-counting dimension, information dimension)
Embryonic body mass
Embryonic body development (body area, percentage cephalic length)
Vasculogenesis and angiogenesis (inferred from YSM vascular network assessment)

Limitations

Small sample size (30 eggs total; 10 per group)
Only two magnetic field intensities tested (0.16 and 0.65 mT)
Single frequency (60 Hz)
Exposure duration limited to 6 h

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 6e-05,
    "sar_wkg": null,
    "duration": "6 h"
  },
  "population": "Japanese quail (Coturnix japonica) embryos/eggs",
  "sample_size": 30,
  "outcomes": [
    "Yolk sac membrane (YSM) vascular network fractal dimensions (box-counting dimension, information dimension)",
    "Embryonic body mass",
    "Embryonic body development (body area, percentage cephalic length)",
    "Vasculogenesis and angiogenesis (inferred from YSM vascular network assessment)"
  ],
  "main_findings": "Japanese quail embryos were exposed to a 60 Hz magnetic field at 0.16 mT or 0.65 mT (6 h). Fractal dimension measures of the yolk sac membrane vascular network and embryonic body mass did not differ among groups, but embryos exposed to 0.65 mT showed reduced body development (smaller body area and percentage cephalic length).",
  "effect_direction": "mixed",
  "limitations": [
    "Small sample size (30 eggs total; 10 per group)",
    "Only two magnetic field intensities tested (0.16 and 0.65 mT)",
    "Single frequency (60 Hz)",
    "Exposure duration limited to 6 h"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "Japanese quail",
    "Coturnix japonica",
    "embryo",
    "yolk sac membrane",
    "vascular network",
    "vasculogenesis",
    "angiogenesis",
    "fractal dimension",
    "extremely low frequency",
    "ELF",
    "magnetic field",
    "60 Hz",
    "0.16 mT",
    "0.65 mT"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "Study involves 60 Hz magnetic field exposure, relevant to ELF exposure contexts, though not explicitly occupational."
    }
  ]
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/15368378.2020.1821708
PubMed: 32954862

Read the original paper →

Related papers

← Back to the Research Library