Generation and propagation of yeast prion are elevated under ELF and 2.0 GHz RF EMF

2017 · Harm · Low evidence

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HarmLow evidencePaper 2206

Study snapshot

Publicationmanual · 2017

Study typeanimal

Reported directionHarm

Evidence ratingLow

Abstract

Generation and propagation of yeast prion are elevated under ELF and 2.0 GHz RF EMF Lian HY, Lin KW, Yang C, Cai P. Generation and propagation of yeast prion [URE3] are elevated under electromagnetic field. Cell Stress Chaperones. 2017 Dec 6. doi: 10.1007/s12192-017-0867-9. Abstract In this study, we studied the effect of 2.0 GHz radio frequency electromagnetic field (RF-EMF) and 50 Hz extremely low frequency electromagnetic field (ELF-EMF) exposure on prion generation and propagation using two budding yeast strains, NT64C and SB34, as model organisms. Under exposure to RF-EMF or ELF-EMF, the de novo generation and propagation of yeast prions [URE3] were elevated in both strains. The elevation increased over time, and the effects of ELF-EMF occurred in a dose-dependent manner. The transcription and expression levels of the molecular chaperones Hsp104, Hsp70-Ssa1/2, and Hsp40-Ydj1 were not statistically significantly changed after exposure. Furthermore, the levels of ROS, as well as the activities of superoxide dismutase (SOD) and catalase (CAT), were significantly elevated after short-term, but not long-term exposure. This work demonstrated for the first time that EMF exposure could elevate the de novo generation and propagation of yeast prions and supports the hypothesis that ROS may play a role in the effects of EMF on protein misfolding. The effects of EMF on protein folding and ROS levels may mediate the broad effects of EMF on cell function. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionharm

PopulationBudding yeast strains (NT64C and SB34)

Sample sizeNot supplied

ExposureELF and RF · 2000 MHz

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Exposure to 2.0 GHz RF-EMF or 50 Hz ELF-EMF was associated with elevated de novo generation and propagation of yeast prion [URE3] in two budding yeast strains, with increases over time and dose-dependent effects reported for ELF-EMF. Chaperone transcription/expression (Hsp104, Hsp70-Ssa1/2, Hsp40-Ydj1) was not statistically significantly changed, while ROS levels and SOD/CAT activities were significantly elevated after short-term but not long-term exposure.

Outcomes measured

De novo generation of yeast prion [URE3]
Propagation of yeast prion [URE3]
Transcription/expression of molecular chaperones (Hsp104, Hsp70-Ssa1/2, Hsp40-Ydj1)
Reactive oxygen species (ROS) levels
Superoxide dismutase (SOD) activity
Catalase (CAT) activity

Limitations

No exposure metrics (e.g., field strength, SAR) reported in the abstract
Sample size not reported in the abstract
Duration definitions for “short-term” and “long-term” not specified in the abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF and RF",
    "source": null,
    "frequency_mhz": 2000,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Budding yeast strains (NT64C and SB34)",
  "sample_size": null,
  "outcomes": [
    "De novo generation of yeast prion [URE3]",
    "Propagation of yeast prion [URE3]",
    "Transcription/expression of molecular chaperones (Hsp104, Hsp70-Ssa1/2, Hsp40-Ydj1)",
    "Reactive oxygen species (ROS) levels",
    "Superoxide dismutase (SOD) activity",
    "Catalase (CAT) activity"
  ],
  "main_findings": "Exposure to 2.0 GHz RF-EMF or 50 Hz ELF-EMF was associated with elevated de novo generation and propagation of yeast prion [URE3] in two budding yeast strains, with increases over time and dose-dependent effects reported for ELF-EMF. Chaperone transcription/expression (Hsp104, Hsp70-Ssa1/2, Hsp40-Ydj1) was not statistically significantly changed, while ROS levels and SOD/CAT activities were significantly elevated after short-term but not long-term exposure.",
  "effect_direction": "harm",
  "limitations": [
    "No exposure metrics (e.g., field strength, SAR) reported in the abstract",
    "Sample size not reported in the abstract",
    "Duration definitions for “short-term” and “long-term” not specified in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "RF-EMF",
    "2.0 GHz",
    "50 Hz",
    "yeast",
    "prion",
    "URE3",
    "protein misfolding",
    "ROS",
    "Hsp104",
    "Hsp70",
    "Hsp40",
    "SOD",
    "catalase"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s12192-017-0867-9
PubMed: Not supplied

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