Significant Cellular Viability Dependence on Time Exposition at ELF-EMF and RF-EMF In Vitro Studies

2019 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 1792

In NIH/3T3 fibroblasts exposed to RF-EMF at 2.54 GHz, cell viability showed significant time dependence, decreasing with exposure time and reaching a reported 71.12% decline after 21 h. In glioblastomas exposed to a 100 µT ELF-EMF, effects on viability were r…

Study snapshot

Publicationmanual · 2019

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Significant Cellular Viability Dependence on Time Exposition at ELF-EMF and RF-EMF In Vitro Studies García-Minguillán López O, Jiménez Valbuena A, 2 Maestú Unturbe C. Significant Cellular Viability Dependence on Time Exposition at ELF-EMF and RF-EMF In Vitro Studies. Int. J. Environ. Res. Public Health 2019, 16(12), 2085; doi.org. Abstract The human concern about the effect of electromagnetic fields (EMFs) has changed over time from the effects produced by EMFs of extremely low frequencies (ELFs) to the effects produced by exposure to a radio frequency (RF), with concerns shifting toward EMFs due to the development of new technologies and forms of communication. Previous studies have analysed the effects produced at different frequencies without considering in detail the effect of the time of exposure. Therefore, in the present study, we analysed in vitro the effect produced by a 100 µT EMF at different ELFs and exposure times in glioblastomas, as well as the effect produced in a fibroblast by an RF-EMF of 2.54 GHz. Our results indicate a significant time dependence in cell viability of fibroblasts exposed to an RF-EMF of 2.54 GHz and a non-time-dependent effect in cell viability of glioblastomas exposed to an ELF-EMF, highlighting the possible relation between frequency and time of exposure. Conclusions In most of the in vitro studies that have been done with EMFs, the time of exposure has not been considered in detail, even though cellular responses, such as cell proliferation, viability and death, clearly vary between chronic and acute exposure. Our results demonstrate a time dependence of NIH/3T3 cell viability when exposed to an RF-EMF of 2.54 GHz, which decreases with time to obtain a 71.12% decline in viability after 21 h of exposure. On the other hand, our results for an ELF-EMF of 100 µT have demonstrated non-time-dependent effects. Frequency dependence effects were observed, obtaining a primary increase in cell viability at 50 Hz and a reduction in cell viability at 30 Hz, showing that even the recommended exposure level affects cell viability. Therefore, the relationship between the frequency and time of exposure could determine the cellular response, demonstrating a need for further research. Open access paper: mdpi.com

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationIn vitro cell lines: glioblastomas and NIH/3T3 fibroblasts

Sample sizeNot supplied

ExposureELF and RF · 2540 MHz · up to 21 h (RF exposure); exposure times varied (ELF and RF)

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

cell viability
cell proliferation (mentioned)
cell death (mentioned)

Limitations

Sample size and experimental replication not reported in the provided abstract/metadata
Specific glioblastoma cell line(s) not specified in the provided abstract/metadata
RF exposure metric (e.g., SAR) not reported in the provided abstract/metadata
Details of exposure setup, controls, and statistical methods not provided in the provided abstract/metadata

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF and RF",
    "source": null,
    "frequency_mhz": 2540,
    "sar_wkg": null,
    "duration": "up to 21 h (RF exposure); exposure times varied (ELF and RF)"
  },
  "population": "In vitro cell lines: glioblastomas and NIH/3T3 fibroblasts",
  "sample_size": null,
  "outcomes": [
    "cell viability",
    "cell proliferation (mentioned)",
    "cell death (mentioned)"
  ],
  "main_findings": "In NIH/3T3 fibroblasts exposed to RF-EMF at 2.54 GHz, cell viability showed significant time dependence, decreasing with exposure time and reaching a reported 71.12% decline after 21 h. In glioblastomas exposed to a 100 µT ELF-EMF, effects on viability were reported as non-time-dependent, with frequency-dependent differences (increase at 50 Hz and reduction at 30 Hz).",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size and experimental replication not reported in the provided abstract/metadata",
    "Specific glioblastoma cell line(s) not specified in the provided abstract/metadata",
    "RF exposure metric (e.g., SAR) not reported in the provided abstract/metadata",
    "Details of exposure setup, controls, and statistical methods not provided in the provided abstract/metadata"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "RF-EMF",
    "in vitro",
    "cell viability",
    "glioblastoma",
    "NIH/3T3",
    "fibroblast",
    "2.54 GHz",
    "100 µT",
    "exposure time",
    "frequency dependence"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3390/ijerph16122085
PubMed: Not supplied

Read the original paper →

Related papers

← Back to the Research Library