Extremely low frequency variable electromagnetic fields affect cancer and noncancerous cells in vitro differently: Preliminary study.

Electromagnetic biology and medicine · 2018 · Mixed · Very low evidence

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MixedVery low evidencePaper 5551

Cancer (U87-MG; 143B) and noncancerous (BJ; HEK) cells exposed to ELF-EMF (2, 20, 30, 50, 60 Hz; sinusoidal/square/triangular; 0.5–3 h) showed different effects on cell viability compared with sham exposure.

Study snapshot

PublicationElectromagnetic biology and medicine · 2018
Study typein_vitro
Reported directionMixed
Evidence ratingVery low

Abstract

The exposure to extremely low frequency electromagnetic field (ELF-EMF) may result in various changes at the cellular level. To identify the effect of ELF-EMF exposure on viability of cells, cancer cells (U87-MG; 143B) and noncancerous cells (BJ; HEK) in exponential growth phase were exposed or sham-exposed to different values of frequency (2, 20, 30, 50 and 60 Hz), different shapes (sinusoidal, square and triangular) and time of exposure (0.5, 1, 2, 3 h) to electromagnetic field. After exposure, viability of cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). We found a different effect of exposition of cancer and noncancerous cells to ELF-EMF on viability of cells. This preliminary study revealed that electro magentic field(EMF) might serve as a potential tool for manipulating viability of cells.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationIn vitro cell lines: cancer (U87-MG; 143B) and noncancerous (BJ; HEK) cells in exponential growth phase
Sample sizeNot supplied
ExposureELF · 0.5, 1, 2, 3 h
Evidence strengthVery lowConfidence: 74% · Peer reviewed: yes

Main findings

Cancer (U87-MG; 143B) and noncancerous (BJ; HEK) cells exposed to ELF-EMF (2, 20, 30, 50, 60 Hz; sinusoidal/square/triangular; 0.5–3 h) showed different effects on cell viability compared with sham exposure.

Outcomes measured

  • Cell viability (MTT assay)

Limitations

  • Preliminary study
  • In vitro design; findings may not translate to in vivo or human health outcomes
  • Sample size and exposure intensity not reported in abstract
  • Direction/magnitude of viability changes not specified in abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "0.5, 1, 2, 3 h"
  },
  "population": "In vitro cell lines: cancer (U87-MG; 143B) and noncancerous (BJ; HEK) cells in exponential growth phase",
  "sample_size": null,
  "outcomes": [
    "Cell viability (MTT assay)"
  ],
  "main_findings": "Cancer (U87-MG; 143B) and noncancerous (BJ; HEK) cells exposed to ELF-EMF (2, 20, 30, 50, 60 Hz; sinusoidal/square/triangular; 0.5–3 h) showed different effects on cell viability compared with sham exposure.",
  "effect_direction": "mixed",
  "limitations": [
    "Preliminary study",
    "In vitro design; findings may not translate to in vivo or human health outcomes",
    "Sample size and exposure intensity not reported in abstract",
    "Direction/magnitude of viability changes not specified in abstract"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF-EMF",
    "electromagnetic field",
    "in vitro",
    "cell viability",
    "MTT",
    "cancer cells",
    "noncancerous cells",
    "U87-MG",
    "143B",
    "BJ",
    "HEK",
    "sham exposure",
    "frequency",
    "waveform"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Study examines extremely low frequency (2–60 Hz) electromagnetic field exposure effects on cells."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/15368378.2017.1408021
PubMed: 29513614

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