Assessing the effect of selenium on cyclin D1 level and nuclear factor kappa b activity in NIH/3T3 fibroblast cells at 2100 MHz electromagnetic field exposure.

Electromagnetic biology and medicine · 2023 · Mixed · Low evidence

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MixedLow evidencePaper 5777

In NIH/3T3 fibroblast cells exposed to 2100 MHz EMF, cell viability percentage was reduced while cyclin D1 level and NF-κB activity increased. Supplementation with 200 nM selenium decreased the effects of 2100 MHz EMF on these parameters.

Study snapshot

PublicationElectromagnetic biology and medicine · 2023

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Although there are numerous studies on the health impacts of electromagnetic field (EMF) of mobile phone operation frequency 2100 MHz, the published works present contradicting results. Long-term exposure to mobile phone frequencies has unclear health hazards. Therefore, it is important to investigate the molecular mechanism of possible biological effects in mobile phone exposure and to determine the corresponding biological markers. Towards this end, this study was designed to assess the effect of 200 nM selenium (Se) on cell viability% [trypan blue], cell cycle biomarker [cyclin D1] and the transcription factor [nuclear factor kappa b (NF-κB)] in NIH/3T3 fibroblast cells when exposed to 2100 MHz mobile phone frequency. When 2100 MHz EMF was exposed to NIH/3T3 fibroblast cells, the cell viability% was reduced, whereas cyclin D1 level and NF-kB activity increased. Also we show that Se supplementation decreases the effects of 2100 MHz EMF on these parameters. Although future studies will be required to investigate the biological effects of EMF emitted by mobile phones, the results obtained here provide an insight into the molecular mechanisms and specifically underlying selenium's protective effect against 2100 MHz EMF exposure.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationNIH/3T3 fibroblast cells

Sample sizeNot supplied

ExposureRF · 2100 MHz · long-term

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

cell viability (trypan blue)
cyclin D1 level
NF-κB activity

Limitations

Exposure duration and conditions (e.g., SAR/dosimetry) not reported in the abstract
Sample size not reported in the abstract
In vitro cell model; findings may not generalize to humans

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "mobile phone",
    "frequency_mhz": 2100,
    "sar_wkg": null,
    "duration": "long-term"
  },
  "population": "NIH/3T3 fibroblast cells",
  "sample_size": null,
  "outcomes": [
    "cell viability (trypan blue)",
    "cyclin D1 level",
    "NF-κB activity"
  ],
  "main_findings": "In NIH/3T3 fibroblast cells exposed to 2100 MHz EMF, cell viability percentage was reduced while cyclin D1 level and NF-κB activity increased. Supplementation with 200 nM selenium decreased the effects of 2100 MHz EMF on these parameters.",
  "effect_direction": "mixed",
  "limitations": [
    "Exposure duration and conditions (e.g., SAR/dosimetry) not reported in the abstract",
    "Sample size not reported in the abstract",
    "In vitro cell model; findings may not generalize to humans"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "2100 MHz",
    "RF-EMF",
    "mobile phone",
    "selenium",
    "NIH/3T3",
    "fibroblast",
    "cell viability",
    "cyclin D1",
    "NF-κB"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/15368378.2023.2252457
PubMed: 37638990

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