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Low intensity microwave radiation as modulator of the L-lactate dehydrogenase activity.

Medical & biological engineering & computing · 2011 · Benefit · Low evidence

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BenefitLow evidencePaper 6498

LDH enzyme solutions irradiated in a TEM cell showed selectively increased LDH activity at 500 MHz (electric field 0.02–2.1 V/m; 1.2×10^-6 to 1.2×10^-2 W/m^2) and at 900 MHz (electric field 0.021–0.21 V/m; 1.2×10^-4 W/m^2), compared with non-irradiated enzyme.

Study snapshot

PublicationMedical & biological engineering & computing · 2011
Study typein_vitro
Reported directionBenefit
Evidence ratingLow

Abstract

In this study, we investigated experimentally the possibility of modulating protein activity by low intensity microwaves by measuring alternations of L: -Lactate Dehydrogenase enzyme (LDH) activity. The LDH enzyme solutions were irradiated by microwaves of the selected frequencies and powers using the Transverse Electro-Magnetic (TEM) cell. The kinetics of the irradiated LDH was measured by continuous monitoring of nicotine adenine dinucleotide, reduced (NADH) absorbance at 340 nm. A comparative analysis of changes in the activity of the irradiated LDH enzyme versus the non-radiated enzyme was performed for the selected frequencies and powers. It was found that LDH activity can be selectively increased only by irradiation at the particular frequencies of 500 MHz [electric field: 0.02 V/m (1.2 × 10⁻⁶ W/m²)-2.1 V/m (1.2 × 10⁻² W/m²)] and 900 MHz [electric field: 0.021-0.21 V/m (1.2 × 10⁻⁴ W/m²)]. Based on results obtained it was concluded that LDH enzyme activity can be modulated by specific frequencies of low power microwave radiation. This finding can serve to support the hypothesis that low intensity microwaves can induce non-thermal effects in bio-molecules.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationNot supplied
Sample sizeNot supplied
ExposureRF
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

LDH enzyme solutions irradiated in a TEM cell showed selectively increased LDH activity at 500 MHz (electric field 0.02–2.1 V/m; 1.2×10^-6 to 1.2×10^-2 W/m^2) and at 900 MHz (electric field 0.021–0.21 V/m; 1.2×10^-4 W/m^2), compared with non-irradiated enzyme.

Outcomes measured

  • L-lactate dehydrogenase (LDH) enzyme activity
  • NADH absorbance at 340 nm (kinetics measure)

Limitations

  • Exposure duration not reported in abstract
  • Sample size/number of replicates not reported in abstract
  • Only enzyme solutions studied (in vitro), limiting generalizability to organisms/health outcomes
  • Frequencies/powers tested beyond those reported are not described in abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "L-lactate dehydrogenase (LDH) enzyme activity",
    "NADH absorbance at 340 nm (kinetics measure)"
  ],
  "main_findings": "LDH enzyme solutions irradiated in a TEM cell showed selectively increased LDH activity at 500 MHz (electric field 0.02–2.1 V/m; 1.2×10^-6 to 1.2×10^-2 W/m^2) and at 900 MHz (electric field 0.021–0.21 V/m; 1.2×10^-4 W/m^2), compared with non-irradiated enzyme.",
  "effect_direction": "benefit",
  "limitations": [
    "Exposure duration not reported in abstract",
    "Sample size/number of replicates not reported in abstract",
    "Only enzyme solutions studied (in vitro), limiting generalizability to organisms/health outcomes",
    "Frequencies/powers tested beyond those reported are not described in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "radiofrequency",
    "low intensity",
    "non-thermal effects",
    "L-lactate dehydrogenase",
    "LDH",
    "enzyme activity",
    "TEM cell",
    "500 MHz",
    "900 MHz",
    "NADH absorbance"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s11517-010-0690-2
PubMed: 21308416

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