Anticancer effects on leiomyosarcoma-bearing Wistar rats after electromagnetic radiation of resonant radiofrequencies.

Hellenic journal of nuclear medicine · 2007 · Benefit · Low evidence

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BenefitLow evidencePaper 5053

Study snapshot

PublicationHellenic journal of nuclear medicine · 2007

Study typeanimal

Reported directionBenefit

Evidence ratingLow

Abstract

In the present study, the effects of a resonant low intensity static electromagnetic field (EMF), causing no thermal effects, on Wistar rats have been investigated. Sarcoma cell lines were isolated from leiomyosarcoma tumors induced in Wistar rats by the subcutaneous (s.c) injection of 3,4-benzopyrene. Furthermore, smooth muscle cells (SMC) were isolated from the aorta of Wistar rats and cultivated. Either leiomyosarcoma cells (LSC) or SMC were used to record a number of characteristic resonant radiofrequencies, in order to determine the specific electromagnetic fingerprint spectrum for each cell line. These spectra were used to compose an appropriate algorithm, which transforms the recorded radiofrequencies to emitted ones. The isolated LSC were cultured and then exposed to a resonant low intensity radiofrequency EMF (RF-EMF), at frequencies between 10 kHz to 120 kHz of the radiowave spectrum. The exposure lasted 45 consecutive minutes daily, for two consecutive days. Three months old female Wistar rats were inoculated with exposed and non-exposed to EMF LSC (4 x 10(6) LCS for animal). Inoculated with non-exposed to EMF cells animals were then randomly separated into three Groups. The first Group was sham exposed to the resonant EMF (control Group-CG), the second Group after the inoculation of LSC and appearance of a palpable tumor mass, was exposed to a non-resonant EMF radiation pattern, for 5 h per day till death of all animals (experimental control Group-ECG). The third Group of animals after inoculation of LSC and the appearance of a palpable tumor mass, was exposed to the resonant EMF radiation for 5 h per day, for a maximum of 60 days (experimental Group-I, EG-I). A fourth Group of animals was inoculated with LSC exposed to EMF irradiation and were not further exposed to irradiation (experimental Group-II, EG-II). Tumor induction was 100% in all Groups studied and all tumors were histologically identified as leiomyosarcomas. In the case of the EG-I, a number of tumors were completely regretted (final tumor induction: 66%). Both Groups of animals inoculated with exposed or non-exposed to the EMF LSC, (EG-I and EG-II, respectively) demonstrated a significant prolongation of the survival time and a lower tumor growth rate, in comparison to the control Group (CG) and the experimental control Group (ECG). However, the survival time of EG-I animals was found to be significantly longer and tumor growth rate significantly lower compared to EG-II animals. In conclusion, our results indicate a specific anticancer effect of resonant EMF irradiation. These results may possibly be attributed to (a) the duration of exposure of LSC and (b) the exposure of the entire animal to this irradiation.

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AI evidence extraction

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Study typeanimal

Effect directionbenefit

Population3-month-old female Wistar rats with leiomyosarcoma (tumors induced via inoculation of leiomyosarcoma cells derived from benzopyrene-induced tumors)

Sample sizeNot supplied

ExposureELF · In vitro: 45 min daily for 2 consecutive days (LSC exposure). In vivo: 5 h/day after palpable tumor mass; resonant exposure up to 60 days (max) or non-resonant exposure 5 h/day until death (group-dependent).

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Tumor induction was 100% in all groups and tumors were histologically leiomyosarcomas. Animals exposed to resonant EMF irradiation after tumor appearance (EG-I) and animals inoculated with EMF-exposed tumor cells (EG-II) showed significantly prolonged survival and lower tumor growth rate versus sham-exposed controls and a non-resonant EMF pattern group. EG-I had significantly longer survival and lower tumor growth rate than EG-II; in EG-I, some tumors completely regressed (final tumor induction 66%).

Outcomes measured

Tumor induction rate
Tumor regression (complete regression in some animals)
Survival time
Tumor growth rate

Limitations

No sample sizes reported in the abstract.
Exposure intensity/field strength and dosimetry (e.g., SAR) not reported.
Randomization details only briefly mentioned; blinding not described.
Frequencies described as 10–120 kHz (low-frequency range) despite being referred to as radiofrequencies; exposure characterization is limited in the abstract.

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "In vitro: 45 min daily for 2 consecutive days (LSC exposure). In vivo: 5 h/day after palpable tumor mass; resonant exposure up to 60 days (max) or non-resonant exposure 5 h/day until death (group-dependent)."
  },
  "population": "3-month-old female Wistar rats with leiomyosarcoma (tumors induced via inoculation of leiomyosarcoma cells derived from benzopyrene-induced tumors)",
  "sample_size": null,
  "outcomes": [
    "Tumor induction rate",
    "Tumor regression (complete regression in some animals)",
    "Survival time",
    "Tumor growth rate"
  ],
  "main_findings": "Tumor induction was 100% in all groups and tumors were histologically leiomyosarcomas. Animals exposed to resonant EMF irradiation after tumor appearance (EG-I) and animals inoculated with EMF-exposed tumor cells (EG-II) showed significantly prolonged survival and lower tumor growth rate versus sham-exposed controls and a non-resonant EMF pattern group. EG-I had significantly longer survival and lower tumor growth rate than EG-II; in EG-I, some tumors completely regressed (final tumor induction 66%).",
  "effect_direction": "benefit",
  "limitations": [
    "No sample sizes reported in the abstract.",
    "Exposure intensity/field strength and dosimetry (e.g., SAR) not reported.",
    "Randomization details only briefly mentioned; blinding not described.",
    "Frequencies described as 10–120 kHz (low-frequency range) despite being referred to as radiofrequencies; exposure characterization is limited in the abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "resonant electromagnetic field",
    "low intensity",
    "non-thermal",
    "10 kHz to 120 kHz",
    "leiomyosarcoma",
    "Wistar rats",
    "tumor growth",
    "survival",
    "tumor regression",
    "non-resonant pattern",
    "sham exposure"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 17684584

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