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[Influence of microwave radiation on synapsin I expression in PC12 cells and its mechanism].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2008 · Mixed · Low evidence

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MixedLow evidencePaper 6470

PC12 cells exposed to microwave radiation (average power density 30 mW/cm^2) showed decreased release of Asp, Glu, GABA and Gly at 1 h. Synapsin I protein decreased at 9 h–2 d, with time-varying changes in synapsin I mRNA and phosphorylation; BDNF and TrkB ex…

Study snapshot

PublicationXi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2008
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

AIM: To investigate the effect of microwave radiation on expression and phosphorylation of synapsin I and to discover the mechanism by research on the change of expression of BDNF and its receptor, TrkB. METHODS: PC12 cells were exposed to microwave with average power density being 30 mW/cm(2). HPLC was used to detect the release of amino acids; RT-PCR, Western blot and immunocytochemistry were used to detect the expressions of synapsin I, BDNF and TrkB; immune co-precipitation was used to study the interaction of BDNF and TrkB. RESULTS: It resulted in the decrease of the release of Asp, Glu, GABA and Gly at 1 h (P<0.01) after radiation. Protein of synapsin I was decreased in 9 h-2 d (P<0.01 or P<0.05); its mRNA was decreased in 3-9 h and increased at 1 d (P<0.01 or P<0.05); its phosphorylation was decreased at 3 h, increased at 1 d, and decreased at 2 d again (P<0.01 or P<0.05) after radiation. Protein of BDNF was decreased at 3 h and increased in 1-2 d (P<0.01 or P<0.05); its mRNA were decreased in 3-9 h, increased at 1d, and decreased at 2 d again (P<0.01 or P<0.05) after radiation. Protein of TrkB was increased in 3 h-1 d (P<0.01 or P<0.05); its mRNA decreased at 3 h and 2 d (P<0.01) after radiation. The interaction between BDNF and TrkB was increased in 3-9 h, but decreased in 1-2 d (P<0.01 or P<0.05) after radiation. CONCLUSION: Microwave radiation can induce the decrease of the release of amino acids and the expression and phosphorylation of synapsin I, and the abnormality of expressions and interaction of BDNF and TrkB in PC12 cells. The factors might play a role in the injury and repair of information transmission in PC12 cells.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationPC12 cells
Sample sizeNot supplied
Exposuremicrowave
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

PC12 cells exposed to microwave radiation (average power density 30 mW/cm^2) showed decreased release of Asp, Glu, GABA and Gly at 1 h. Synapsin I protein decreased at 9 h–2 d, with time-varying changes in synapsin I mRNA and phosphorylation; BDNF and TrkB expression and BDNF–TrkB interaction also showed time-dependent alterations after exposure.

Outcomes measured

  • Synapsin I protein expression
  • Synapsin I mRNA expression
  • Synapsin I phosphorylation
  • BDNF protein expression
  • BDNF mRNA expression
  • TrkB protein expression
  • TrkB mRNA expression
  • BDNF–TrkB interaction
  • Release of amino acids (Asp, Glu, GABA, Gly)

Limitations

  • Frequency of the microwave exposure not reported in abstract
  • Exposure duration/regimen not clearly specified beyond post-exposure timepoints
  • No sample size reported in abstract
  • In vitro model (PC12 cells) may not generalize to in vivo or human outcomes
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "PC12 cells",
  "sample_size": null,
  "outcomes": [
    "Synapsin I protein expression",
    "Synapsin I mRNA expression",
    "Synapsin I phosphorylation",
    "BDNF protein expression",
    "BDNF mRNA expression",
    "TrkB protein expression",
    "TrkB mRNA expression",
    "BDNF–TrkB interaction",
    "Release of amino acids (Asp, Glu, GABA, Gly)"
  ],
  "main_findings": "PC12 cells exposed to microwave radiation (average power density 30 mW/cm^2) showed decreased release of Asp, Glu, GABA and Gly at 1 h. Synapsin I protein decreased at 9 h–2 d, with time-varying changes in synapsin I mRNA and phosphorylation; BDNF and TrkB expression and BDNF–TrkB interaction also showed time-dependent alterations after exposure.",
  "effect_direction": "mixed",
  "limitations": [
    "Frequency of the microwave exposure not reported in abstract",
    "Exposure duration/regimen not clearly specified beyond post-exposure timepoints",
    "No sample size reported in abstract",
    "In vitro model (PC12 cells) may not generalize to in vivo or human outcomes"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "PC12 cells",
    "synapsin I",
    "phosphorylation",
    "BDNF",
    "TrkB",
    "amino acid release",
    "RT-PCR",
    "Western blot",
    "immunocytochemistry",
    "co-immunoprecipitation"
  ],
  "suggested_hubs": [
    {
      "slug": "mechanisms-in-vitro",
      "weight": 0.9,
      "reason": "In vitro study examining molecular/mechanistic endpoints (synapsin I, BDNF/TrkB) after microwave exposure."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 18616904

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