Study of Electrical Stimulation with Different Electric Field Intensities in Regulating Differentiation of PC12 Cells

2018 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 2030

Study snapshot

Publicationmanual · 2018

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Study of Electrical Stimulation with Different Electric Field Intensities in Regulating Differentiation of PC12 Cells Jing W, Zhang Y, Cai Q, Chen G, Wang L, Yang X, Zhong W. Study of Electrical Stimulation with Different Electric Field Intensities in Regulating Differentiation of PC12 Cells. ACS Chem Neurosci. 2018 Sep 13. doi: 10.1021/acschemneuro.8b00286. Abstract The strategy of using electrical stimulation (ES) to promote neural differentiation and regeneration of injured nerves is proven feasible. The study on the possible molecular mechanisms in relation to this ES promotion effect should be helpful to understand the phenomenon. In this study, it was identified the neuronal differentiation of PC12 cells was enhanced when the electric field intensity was in the range of 30-80 mV/mm, lower or higher electric field intensity displayed inferior effect. Under ES, however, levels of intracellular reactive oxygen species (ROS), intracellular Ca2+ dynamics and expression of TREK-1 were measured gradually increasing alongside higher electric field intensity. Trying to understand the relationship between the ES enhancement on differentiation and these variations in cell activities, parallel experiments were conducted by introducing exogeneous H2O2 into culture systems at different concentrations. Similarly, the effects of H2O2 concentration on neuronal differentiation of PC12 cells, intracellular ROS and Ca2+ levels, and TREK-1 expression were systematically characterized. In comparative studies, it was found the two cases that ES of 50 mV/mm for 2 h/day and H2O2 of 5 µM in culture medium shared comparable results in intracellular ROS and Ca2+ levels, and TREK-1 expression. Higher H2O2 concentration (e.g. 10 µM and 20 µM) demonstrated adverse effect on cell differentiation and caused DNA damage. A stronger ES (e.g. 100 mV/mm), being associated with higher intracellular ROS level, also resulted in weaker enhancement on the neuronal differentiation of PC12 cells. These facts suggested that the intracellular ROS generated under ES might be an intermediate signal transducer involved in cascade reactions relative to cell differentiation. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationPC12 cells

Sample sizeNot supplied

Exposure2 h/day

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Neuronal differentiation of PC12 cells was enhanced with electrical stimulation when electric field intensity was 30–80 mV/mm; lower or higher intensities showed inferior effects. With increasing field intensity, intracellular ROS, Ca2+ dynamics, and TREK-1 expression increased; stronger stimulation (e.g., 100 mV/mm) was associated with higher ROS and weaker enhancement of differentiation. Exogenous H2O2 at 5 µM produced comparable ROS/Ca2+/TREK-1 results to 50 mV/mm ES for 2 h/day, while higher H2O2 (10–20 µM) adversely affected differentiation and caused DNA damage.

Outcomes measured

neuronal differentiation
intracellular reactive oxygen species (ROS)
intracellular Ca2+ dynamics/levels
TREK-1 expression
DNA damage

Limitations

Sample size not reported in abstract
Frequency/waveform characteristics of electrical stimulation not described in abstract
In vitro cell model; generalizability to organisms/humans not addressed in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "electrical stimulation",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "2 h/day"
  },
  "population": "PC12 cells",
  "sample_size": null,
  "outcomes": [
    "neuronal differentiation",
    "intracellular reactive oxygen species (ROS)",
    "intracellular Ca2+ dynamics/levels",
    "TREK-1 expression",
    "DNA damage"
  ],
  "main_findings": "Neuronal differentiation of PC12 cells was enhanced with electrical stimulation when electric field intensity was 30–80 mV/mm; lower or higher intensities showed inferior effects. With increasing field intensity, intracellular ROS, Ca2+ dynamics, and TREK-1 expression increased; stronger stimulation (e.g., 100 mV/mm) was associated with higher ROS and weaker enhancement of differentiation. Exogenous H2O2 at 5 µM produced comparable ROS/Ca2+/TREK-1 results to 50 mV/mm ES for 2 h/day, while higher H2O2 (10–20 µM) adversely affected differentiation and caused DNA damage.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Frequency/waveform characteristics of electrical stimulation not described in abstract",
    "In vitro cell model; generalizability to organisms/humans not addressed in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electrical stimulation",
    "electric field intensity",
    "PC12 cells",
    "neuronal differentiation",
    "ROS",
    "Ca2+",
    "TREK-1",
    "H2O2",
    "DNA damage"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1021/acschemneuro.8b00286
PubMed: Not supplied

Read the original paper →

Related papers

← Back to the Research Library