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Effect of acute millimeter wave exposure on dopamine metabolism of NGF-treated PC12 cells

2017 · No effect · Low evidence

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No effectLow evidencePaper 1337

After 24 h exposure to 60.4 GHz millimeter waves at an incident power density of 5 mW/cm², dopamine turnover (DOPAC/DA ratio and DOPAC accumulation) and DAT protein expression were not impacted in NGF-treated PC12 cells. Extracellular DOPAC accumulation was s…

Study snapshot

Publicationmanual · 2017
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

Effect of acute millimeter wave exposure on dopamine metabolism of NGF-treated PC12 cells Haas AJ, Le Page Y, Zhadobov M, Sauleau R, Dréan YL, Saligaut C. Effect of acute millimeter wave exposure on dopamine metabolism of NGF-treated PC12 cells. J Radiat Res. 2017 Feb 24:1-7. doi: 10.1093/jrr/rrx004. Abstract Several forthcoming wireless telecommunication systems will use electromagnetic frequencies at millimeter waves (MMWs), and technologies developed around the 60-GHz band will soon know a widespread distribution. Free nerve endings within the skin have been suggested to be the targets of MMW therapy which has been used in the former Soviet Union. So far, no studies have assessed the impact of MMW exposure on neuronal metabolism. Here, we investigated the effects of a 24-h MMW exposure at 60.4 GHz, with an incident power density (IPD) of 5 mW/cm², on the dopaminergic turnover of NGF-treated PC12 cells. After MMW exposure, both intracellular and extracellular contents of dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) were studied using high performance liquid chromatography. Impact of exposure on the dopamine transporter (DAT) expression was also assessed by immunocytochemistry. We analyzed the dopamine turnover by assessing the ratio of DOPAC to DA, and measuring DOPAC accumulation in the medium. Neither dopamine turnover nor DAT protein expression level were impacted by MMW exposure. However, extracellular accumulation of DOPAC was found to be slightly increased, but not significantly. This result was related to the thermal effect, and overall, no evidence of non-thermal effects of MMW exposure were observed on dopamine metabolism. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationNGF-treated PC12 cells
Sample sizeNot supplied
ExposuremmWave · 60400 MHz · 24 h
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

After 24 h exposure to 60.4 GHz millimeter waves at an incident power density of 5 mW/cm², dopamine turnover (DOPAC/DA ratio and DOPAC accumulation) and DAT protein expression were not impacted in NGF-treated PC12 cells. Extracellular DOPAC accumulation was slightly increased but not significantly, and was related to thermal effects; no evidence of non-thermal effects on dopamine metabolism was observed.

Outcomes measured

  • Intracellular dopamine (DA) content
  • Extracellular dopamine (DA) content
  • Intracellular 3,4-dihydroxyphenylacetic acid (DOPAC) content
  • Extracellular DOPAC content
  • Dopamine turnover (DOPAC/DA ratio; DOPAC accumulation in medium)
  • Dopamine transporter (DAT) protein expression (immunocytochemistry)
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "mmWave",
    "source": null,
    "frequency_mhz": 60400,
    "sar_wkg": null,
    "duration": "24 h"
  },
  "population": "NGF-treated PC12 cells",
  "sample_size": null,
  "outcomes": [
    "Intracellular dopamine (DA) content",
    "Extracellular dopamine (DA) content",
    "Intracellular 3,4-dihydroxyphenylacetic acid (DOPAC) content",
    "Extracellular DOPAC content",
    "Dopamine turnover (DOPAC/DA ratio; DOPAC accumulation in medium)",
    "Dopamine transporter (DAT) protein expression (immunocytochemistry)"
  ],
  "main_findings": "After 24 h exposure to 60.4 GHz millimeter waves at an incident power density of 5 mW/cm², dopamine turnover (DOPAC/DA ratio and DOPAC accumulation) and DAT protein expression were not impacted in NGF-treated PC12 cells. Extracellular DOPAC accumulation was slightly increased but not significantly, and was related to thermal effects; no evidence of non-thermal effects on dopamine metabolism was observed.",
  "effect_direction": "no_effect",
  "limitations": [],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "millimeter waves",
    "60.4 GHz",
    "incident power density",
    "5 mW/cm²",
    "PC12 cells",
    "NGF-treated",
    "dopamine",
    "DOPAC",
    "dopamine turnover",
    "DAT",
    "thermal effects",
    "non-thermal effects"
  ],
  "suggested_hubs": [
    {
      "slug": "5g-policy",
      "weight": 0.35,
      "reason": "Study examines millimeter-wave exposure in the 60 GHz band, relevant to forthcoming wireless telecommunication systems."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1093/jrr/rrx004
PubMed: Not supplied

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