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Effects of acute and chronic low frequency electromagnetic field exposure on PC12 cells during neuronal differentiation.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2010 · Mixed · Low evidence

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MixedLow evidencePaper 4345

Chronic ELF-EMF exposure (0.1-1.0 mT) did not significantly affect proliferation or neuritogenesis. Acute exposure increased ROS levels and decreased catalase activity, while chronic exposure increased catalase activity. Acute exposure affected intracellular …

Study snapshot

PublicationCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2010
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

BACKGROUND/AIMS: The purpose of this study was to provide information about the in vitro neuritogenesis during cell exposure to extremely low frequency electromagnetic fields (ELF-EMFs) of different intensities and durations using pheochromocytoma-derived cell line (PC12 cells) as neuronal model. METHODS: Proliferative rates and neuritogenesis were tested by colorimetric assay and morphological analysis, respectively; reactive oxygen species (ROS) levels and intracellular Ca(2+) variations monitored using single cell videomicroscopy. RESULTS: The long-lasting ELF-EMF exposure (0.1-1.0 mT) did not appear to significantly affect the biological response (proliferation and neuritogenesis). However, during the acute ELF-EMF exposure (30 min), in undifferentiated PC12 cells, there were increased ROS levels and decreased catalase activity, that, conversely, resulted increased after chronic exposure (7 days) at 1.0 mT. Acute exposure (0.1-1.0 mT) affected the spontaneous intracellular Ca(2+) variations in undifferentiated cells, in which basal intracellular Ca(2+) resulted increased after chronic exposure. In addition acute exposure affected cell response to a depolarizing agent, while basal membrane potential was not changed. CONCLUSION: Even if further studies remain necessary to identify the ROS/intracellular Ca(2+)cross-talking pathway activated by ELF-EMF exposure, we support the hypothesis that ROS and Ca(2+) could be the cellular "primum movens" of the ELF-EMF induced effects on biological systems.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationPC12 cells (pheochromocytoma-derived neuronal model)
Sample sizeNot supplied
ExposureELF · acute (30 min) and chronic (7 days) exposure
Evidence strengthLowConfidence: 40% · Peer reviewed: yes

Main findings

Chronic ELF-EMF exposure (0.1-1.0 mT) did not significantly affect proliferation or neuritogenesis. Acute exposure increased ROS levels and decreased catalase activity, while chronic exposure increased catalase activity. Acute exposure affected intracellular Ca(2+) variations and cell response to depolarizing agent, but basal membrane potential was unchanged.

Outcomes measured

  • proliferation rate
  • neuritogenesis
  • reactive oxygen species (ROS) levels
  • catalase activity
  • intracellular Ca(2+) variations
  • cell response to depolarizing agent
  • basal membrane potential

Limitations

  • In vitro study using a single cell line model
  • No direct measurement of frequency or SAR reported
  • Mechanistic pathways remain to be clarified
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "acute (30 min) and chronic (7 days) exposure"
  },
  "population": "PC12 cells (pheochromocytoma-derived neuronal model)",
  "sample_size": null,
  "outcomes": [
    "proliferation rate",
    "neuritogenesis",
    "reactive oxygen species (ROS) levels",
    "catalase activity",
    "intracellular Ca(2+) variations",
    "cell response to depolarizing agent",
    "basal membrane potential"
  ],
  "main_findings": "Chronic ELF-EMF exposure (0.1-1.0 mT) did not significantly affect proliferation or neuritogenesis. Acute exposure increased ROS levels and decreased catalase activity, while chronic exposure increased catalase activity. Acute exposure affected intracellular Ca(2+) variations and cell response to depolarizing agent, but basal membrane potential was unchanged.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study using a single cell line model",
    "No direct measurement of frequency or SAR reported",
    "Mechanistic pathways remain to be clarified"
  ],
  "evidence_strength": "low",
  "confidence": 0.4,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "neuritogenesis",
    "PC12 cells",
    "reactive oxygen species",
    "intracellular calcium",
    "in vitro"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1159/000324003
PubMed: 21220925

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