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The In Vitro Enhancement of Retinal Cell Viability via mA and mC RNA Methylation-Mediated Changes in the Levels of Heme Oxygenase (HO-1) and DNA Damage Repair Molecules Using a 50 Hz Sinusoidal Electromagnetic Field (EMF).

International journal of molecular sciences · 2024 · Benefit · Low evidence

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BenefitLow evidencePaper 5812

Exposure of ARPE-19 and RGC-5 cells to a 50 Hz, 1.3 mT sinusoidal EMF for 15 or 30 minutes was associated with improved proliferative and migratory capacity. EMF-exposed cells showed elevated HO-1 levels, increased mA and mC status (linked to METTL3 and NSUN2…

Study snapshot

PublicationInternational journal of molecular sciences · 2024
Study typein_vitro
Reported directionBenefit
Evidence ratingLow

Abstract

Degenerative retinal diseases can lead to blindness if left untreated. At present, there are no curative therapies for retinal diseases. Therefore, effective treatment strategies for slowing the progression of retinal diseases and thus improving patients' life standards are urgently needed. The present study aimed to assess the effect of sinusoidal electromagnetic field (EMF) (50 Hz, 1.3 mT) treatment for 15 and 30 min on spontaneously arising retinal pigment epithelial cells (ARPE-19) and retinal ganglion cells (RGC-5) and its short-term post-treatment significance. Our study indicated the beneficial impact of EMF treatment on the proliferative and migratory capacity of the tested cells. ARPE-19 and RGC-5 cells exposed to an EMF exhibited elevated levels of HO-1, increased N6-methyladenosine (mA) and N5-methylcytosine (mC) status mediated by METTL3 and NSUN2, respectively, and changes in levels of DNA damage repair factors, which may contribute to the regenerative properties of ARPE-19 and RGC-5 cells. Overall, this analysis showed that EMF (sinusoidal, 50 Hz, 1.3 mT) treatment may serve as a potential therapeutic strategy for retinal diseases.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationNot supplied
Sample sizeNot supplied
ExposureELF · 0.05 MHz · 15 and 30 min (with short-term post-treatment assessment)
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Exposure of ARPE-19 and RGC-5 cells to a 50 Hz, 1.3 mT sinusoidal EMF for 15 or 30 minutes was associated with improved proliferative and migratory capacity. EMF-exposed cells showed elevated HO-1 levels, increased mA and mC status (linked to METTL3 and NSUN2), and changes in DNA damage repair factor levels.

Outcomes measured

  • Retinal pigment epithelial cell (ARPE-19) proliferative capacity
  • Retinal pigment epithelial cell (ARPE-19) migratory capacity
  • Retinal ganglion cell (RGC-5) proliferative capacity
  • Retinal ganglion cell (RGC-5) migratory capacity
  • Heme oxygenase-1 (HO-1) levels
  • N6-methyladenosine (mA) status (mediated by METTL3)
  • N5-methylcytosine (mC) status (mediated by NSUN2)
  • Levels of DNA damage repair factors
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "15 and 30 min (with short-term post-treatment assessment)"
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Retinal pigment epithelial cell (ARPE-19) proliferative capacity",
    "Retinal pigment epithelial cell (ARPE-19) migratory capacity",
    "Retinal ganglion cell (RGC-5) proliferative capacity",
    "Retinal ganglion cell (RGC-5) migratory capacity",
    "Heme oxygenase-1 (HO-1) levels",
    "N6-methyladenosine (mA) status (mediated by METTL3)",
    "N5-methylcytosine (mC) status (mediated by NSUN2)",
    "Levels of DNA damage repair factors"
  ],
  "main_findings": "Exposure of ARPE-19 and RGC-5 cells to a 50 Hz, 1.3 mT sinusoidal EMF for 15 or 30 minutes was associated with improved proliferative and migratory capacity. EMF-exposed cells showed elevated HO-1 levels, increased mA and mC status (linked to METTL3 and NSUN2), and changes in DNA damage repair factor levels.",
  "effect_direction": "benefit",
  "limitations": [],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "1.3 mT",
    "sinusoidal electromagnetic field",
    "ELF-EMF",
    "ARPE-19",
    "RGC-5",
    "retinal pigment epithelial cells",
    "retinal ganglion cells",
    "cell viability",
    "proliferation",
    "migration",
    "HO-1",
    "heme oxygenase-1",
    "m6A",
    "N6-methyladenosine",
    "m5C",
    "N5-methylcytosine",
    "METTL3",
    "NSUN2",
    "DNA damage repair"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3390/ijms252413606
PubMed: 39769368

Read the original paper →

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