Changes of cationic transport in AtCAX5 transformant yeast by electromagnetic field environments.

Journal of biological physics · 2018 · Mixed · Low evidence

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MixedLow evidencePaper 5532

Study snapshot

PublicationJournal of biological physics · 2018

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

The electromagnetic field (EMF) is newly considered as an exogenous environmental stimulus that is closely related to ion transportation on the cellular membrane, maintaining the internal ionic homeostasis. Cation transports of Ca and other metal ions, Cd, Zn, and Mnwere studied in terms of the external Ca stress, [Ca], and exposure to the physical EMF. A specific yeast strain K667 was used for controlling CAX5 (cation/H exchanger) expression. Culture samples were exposed to 60 Hz, 0.1 mT sinusoidal or square magnetics waves, and intracellular cations of each sample were measured and analyzed. AtCAX5 transformant yeast grew normally under the metallic stress. However, the growth of the control group was significantly inhibited under the same cation concentration; 60 Hz and 0.1 mT magnetic field enhanced intracellular cation concentrations significantly as exposure time increased both in the AtCAX5 transformed yeast and in the control group. However, the AtCAX5-transformed yeast showed higher concentration of the intracellular cations than the control group under the same exposure EMF. AtCAX5-transformed yeasts displayed an increment in [Ca], [K], [Na], and [Zn] concentration under the presence of both sinusoidal and square-waved EMF stresses compared to the control group, which shows that AtCAX5 expressed in the vacuole play an important role in maintaining the homeostasis of intracellular cations. These findings could be utilized in the cultivation of the crops which were resistant to excessive exogenous ions or in the production of biomass containing a large proportion of ions for nutritional food or in the bioremediation process in metal-polluted environments.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionmixed

PopulationYeast strain K667 (AtCAX5 transformant and control)

Sample sizeNot supplied

ExposureELF · 6e-05 MHz

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Culture samples exposed to 60 Hz, 0.1 mT sinusoidal or square magnetic waves showed significantly increased intracellular cation concentrations with increasing exposure time in both AtCAX5-transformed yeast and control yeast. AtCAX5-transformed yeast had higher intracellular cation concentrations than controls under the same EMF exposure and grew normally under metal stress while control growth was significantly inhibited under the same cation concentration.

Outcomes measured

Intracellular cation concentrations (Ca, K, Na, Zn, Cd, Mn)
Yeast growth under metal/cation stress

Limitations

Sample size not reported in abstract
Exposure duration/timepoints not specified in abstract
Study conducted in yeast (non-human model), limiting direct relevance to human health

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": 6e-05,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Yeast strain K667 (AtCAX5 transformant and control)",
  "sample_size": null,
  "outcomes": [
    "Intracellular cation concentrations (Ca, K, Na, Zn, Cd, Mn)",
    "Yeast growth under metal/cation stress"
  ],
  "main_findings": "Culture samples exposed to 60 Hz, 0.1 mT sinusoidal or square magnetic waves showed significantly increased intracellular cation concentrations with increasing exposure time in both AtCAX5-transformed yeast and control yeast. AtCAX5-transformed yeast had higher intracellular cation concentrations than controls under the same EMF exposure and grew normally under metal stress while control growth was significantly inhibited under the same cation concentration.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure duration/timepoints not specified in abstract",
    "Study conducted in yeast (non-human model), limiting direct relevance to human health"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic field",
    "ELF",
    "60 Hz",
    "0.1 mT",
    "magnetic field",
    "yeast",
    "AtCAX5",
    "cation/H exchanger",
    "ion transport",
    "calcium",
    "zinc",
    "sodium",
    "potassium",
    "metal stress"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s10867-018-9500-0
PubMed: 29882183

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