Effects of 50 Hz electromagnetic field exposure on apoptosis and differentiation in a neuroblastoma cell line.

Bioelectromagnetics · 2003 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 5263

Study snapshot

PublicationBioelectromagnetics · 2003

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Experiments were carried out to assess whether a magnetic field of 50 Hz and 1 mT can influence apoptosis and proliferation in the human neuroblastoma cell line LAN-5. TUNEL assays and poly-ADP ribose polymerase (PARP) expression analysis were performed to test apoptosis induction, and the WST-1 assay was used to calculate the proliferation index in a long term exposure. No alterations were found in cellular ability to undergo programmed cell death, but a small increase in the proliferation index was evidenced after 7 days of continuous exposure. Also, a slight and transient increase of B-myb oncogene expression was detected after 5 days of exposure. Combined exposures of cells to EMF and to chemical agents which interfere with proliferation, such as the differentiative agent retinoic acid and the apoptotic inducer camptothecin, showed an antagonistic effect of magnetic fields against the differentiation of the LAN-5 cells and a protective effect towards apoptosis.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman neuroblastoma cell line (LAN-5)

Sample sizeNot supplied

ExposureELF · 0.05 MHz · continuous exposure; effects reported at 5 and 7 days

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In LAN-5 cells exposed to a 50 Hz, 1 mT magnetic field, no alterations were found in the ability to undergo programmed cell death under the apoptosis assays used. A small increase in proliferation index was observed after 7 days of continuous exposure, and a slight transient increase in B-myb expression after 5 days. In combined exposures, magnetic fields showed an antagonistic effect against retinoic-acid-induced differentiation and a protective effect toward camptothecin-induced apoptosis.

Outcomes measured

Apoptosis (TUNEL assay)
PARP expression
Cell proliferation index (WST-1 assay)
B-myb oncogene expression
Differentiation response with retinoic acid (combined exposure)
Apoptosis response with camptothecin (combined exposure)

Limitations

In vitro cell line study; generalizability to humans not addressed in abstract
Sample size not reported in abstract
Magnitude and statistical significance of reported changes not provided in abstract
Exposure duration details beyond 5 and 7 days not fully specified in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "laboratory magnetic field exposure",
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "continuous exposure; effects reported at 5 and 7 days"
  },
  "population": "Human neuroblastoma cell line (LAN-5)",
  "sample_size": null,
  "outcomes": [
    "Apoptosis (TUNEL assay)",
    "PARP expression",
    "Cell proliferation index (WST-1 assay)",
    "B-myb oncogene expression",
    "Differentiation response with retinoic acid (combined exposure)",
    "Apoptosis response with camptothecin (combined exposure)"
  ],
  "main_findings": "In LAN-5 cells exposed to a 50 Hz, 1 mT magnetic field, no alterations were found in the ability to undergo programmed cell death under the apoptosis assays used. A small increase in proliferation index was observed after 7 days of continuous exposure, and a slight transient increase in B-myb expression after 5 days. In combined exposures, magnetic fields showed an antagonistic effect against retinoic-acid-induced differentiation and a protective effect toward camptothecin-induced apoptosis.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell line study; generalizability to humans not addressed in abstract",
    "Sample size not reported in abstract",
    "Magnitude and statistical significance of reported changes not provided in abstract",
    "Exposure duration details beyond 5 and 7 days not fully specified in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "1 mT",
    "ELF magnetic field",
    "LAN-5",
    "neuroblastoma",
    "apoptosis",
    "proliferation",
    "PARP",
    "TUNEL",
    "WST-1",
    "B-myb",
    "retinoic acid",
    "camptothecin",
    "differentiation"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.10130
PubMed: 12955756

Read the original paper →

Related papers

← Back to the Research Library