Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two- Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures

Molecular neurobiology · 2020 · Mixed · Low evidence

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MixedLow evidencePaper 1615

Study snapshot

PublicationMolecular neurobiology · 2020

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two- Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures Claudia Consales, Alessio Butera, Caterina Merla, Emanuela Pasquali, Vanni Lopresto, Rosanna Pinto, Maria Pierdomenico, Mariateresa Mancuso, Carmela Marino, Barbara Benassi. Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three- Dimensional (3D) In Vitro Cultures. Mol Neurobiol. 2020 Nov 24. doi: 10.1007/s12035-020-02192-x. Abstract We here characterize the response to the extremely low-frequency (ELF) magnetic field (MF, 50 Hz, 1 mT) of SH- SY5Y human neuroblastoma cells, cultured in a three-dimensional (3D) Alvetex(r) scaffold compared to conventional two-dimensional (2D) monolayers. We proved that the growing phenotype of proliferating SH-SY5Y cells is not affected by the culturing conditions, as morphology, cell cycle distribution, proliferation/differentiation gene expression of 3D-cultures overlap what reported in 2D plates. In response to 72-h exposure to 50-Hz MF, we demonstrated that no proliferation change and apoptosis activation occur in both 2D and 3D cultures. Consistently, no modulation of Ki67, MYCN, CCDN1, and Nestin, of invasiveness and neo-angiogenesis-controlling genes (HIF-1α, VEGF, and PDGF) and of microRNA epigenetic signature (miR-21-5p, miR-222-3p and miR-133b) is driven by ELF exposure. Conversely, intracellular glutathione content and SOD1 expression are exclusively impaired in 3D-culture cells in response to the MF, whereas no change of such redox modulators is observed in SH-SY5Y cells if grown on 2D monolayers. Moreover, ELF-MF synergizes with the differentiating agents to stimulate neuroblastoma differentiation into a dopaminergic (DA) phenotype in the 3D- scaffold culture only, as growth arrest and induction of p21, TH, DAT, and GAP43 are reported in ELF-exposed SH-SY5Y cells exclusively if grown on 3D scaffolds. As overall, our findings prove that 3D culture is a more reliable experimental model for studying SH-SY5Y response to ELF-MF if compared to 2D conventional monolayer, and put the bases for promoting 3D systems in future studies addressing the interaction between electromagnetic fields and biological systems. pubmed.ncbi.nlm.nih.gov

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Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationSH-SY5Y human neuroblastoma cells (2D monolayers vs 3D Alvetex scaffold cultures)

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 72 h

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

After 72-h exposure to a 50-Hz, 1 mT magnetic field, no changes in proliferation or apoptosis were observed in either 2D or 3D SH-SY5Y cultures, and multiple proliferation/angiogenesis-related genes and selected microRNAs were not modulated. In 3D cultures only, intracellular glutathione content and SOD1 expression were impaired, and ELF-MF synergized with differentiating agents to promote dopaminergic differentiation markers (growth arrest and induction of p21, TH, DAT, GAP43).

Outcomes measured

Cell morphology
Cell cycle distribution
Proliferation
Apoptosis
Gene expression (Ki67, MYCN, CCDN1, Nestin)
Gene expression related to invasiveness/neo-angiogenesis (HIF-1α, VEGF, PDGF)
microRNA expression (miR-21-5p, miR-222-3p, miR-133b)
Intracellular glutathione content
SOD1 expression
Differentiation into dopaminergic phenotype (growth arrest; p21, TH, DAT, GAP43)

Limitations

Sample size not reported in abstract
In vitro model using a neuroblastoma cell line; generalizability to humans not addressed in abstract
Differentiation effects described as synergy with differentiating agents; agent(s) not specified in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "72 h"
  },
  "population": "SH-SY5Y human neuroblastoma cells (2D monolayers vs 3D Alvetex scaffold cultures)",
  "sample_size": null,
  "outcomes": [
    "Cell morphology",
    "Cell cycle distribution",
    "Proliferation",
    "Apoptosis",
    "Gene expression (Ki67, MYCN, CCDN1, Nestin)",
    "Gene expression related to invasiveness/neo-angiogenesis (HIF-1α, VEGF, PDGF)",
    "microRNA expression (miR-21-5p, miR-222-3p, miR-133b)",
    "Intracellular glutathione content",
    "SOD1 expression",
    "Differentiation into dopaminergic phenotype (growth arrest; p21, TH, DAT, GAP43)"
  ],
  "main_findings": "After 72-h exposure to a 50-Hz, 1 mT magnetic field, no changes in proliferation or apoptosis were observed in either 2D or 3D SH-SY5Y cultures, and multiple proliferation/angiogenesis-related genes and selected microRNAs were not modulated. In 3D cultures only, intracellular glutathione content and SOD1 expression were impaired, and ELF-MF synergized with differentiating agents to promote dopaminergic differentiation markers (growth arrest and induction of p21, TH, DAT, GAP43).",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "In vitro model using a neuroblastoma cell line; generalizability to humans not addressed in abstract",
    "Differentiation effects described as synergy with differentiating agents; agent(s) not specified in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF magnetic field",
    "50 Hz",
    "1 mT",
    "SH-SY5Y",
    "neuroblastoma",
    "2D culture",
    "3D culture",
    "Alvetex scaffold",
    "proliferation",
    "apoptosis",
    "oxidative stress",
    "glutathione",
    "SOD1",
    "dopaminergic differentiation",
    "microRNA"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "Study uses an extremely low-frequency (50 Hz) magnetic field exposure relevant to ELF MF research, though no specific occupational setting is described."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s12035-020-02192-x
PubMed: 33230715

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