Power frequency magnetic field promotes a more malignant phenotype in neuroblastoma cells via redox- related mechanisms

2017 · Harm · Low evidence

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HarmLow evidencePaper 2351

Study snapshot

Publicationmanual · 2017

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

Power frequency magnetic field promotes a more malignant phenotype in neuroblastoma cells via redox- related mechanisms Falone S, Santini S Jr, Cordone V, Cesare P, Bonfigli A, Grannonico M, Di Emidio G, Tatone C, Cacchio M, Amicarelli F. Power frequency magnetic field promotes a more malignant phenotype in neuroblastoma cells via redox-related mechanisms.Sci Rep. 2017 Sep 13;7(1):11470. doi: 10.1038/s41598-017-11869-8. Abstract In accordance with the classification of the International Agency for Research on Cancer, extremely low frequency magnetic fields (ELF-MF) are suspected to promote malignant progression by providing survival advantage to cancer cells through the activation of critical cytoprotective pathways. Among these, the major antioxidative and detoxification defence systems might be targeted by ELF-MF by conferring cells significant resistance against clinically-relevant cytotoxic agents. We investigated whether the hyperproliferation that is induced in SH-SY5Y human neuroblastoma cells by a 50 Hz, 1 mT ELF magnetic field was supported by improved defence towards reactive oxygen species (ROS) and xenobiotics, as well as by reduced vulnerability against both H2O2 and anti-tumor ROS-generating drug doxorubicin. ELF-MF induced a proliferative and survival advantage by activating key redox-responsive antioxidative and detoxification cytoprotective pathways that are associated with a more aggressive behavior of neuroblastoma cells. This was coupled with the upregulation of the major sirtuins, as well as with increased signaling activity of the erythroid 2-related nuclear transcription factor 2 (NRF2). Interestingly, we also showed that the exposure to 50 Hz MF as low as 100 µT may still be able to alter behavior and responses of cancer cells to clinically-relevant drugs. ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationSH-SY5Y human neuroblastoma cells

Sample sizeNot supplied

ExposureELF · 0.05 MHz

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In SH-SY5Y human neuroblastoma cells, exposure to a 50 Hz, 1 mT extremely low frequency magnetic field induced hyperproliferation and a proliferative/survival advantage. The abstract reports activation of redox-responsive antioxidative and detoxification cytoprotective pathways (including increased NRF2 signaling and upregulation of sirtuins), reduced vulnerability to H2O2 and doxorubicin, and notes that effects on behavior and drug responses were also observed at 50 Hz as low as 100 µT.

Outcomes measured

Cell proliferation/hyperproliferation
Cell survival advantage
Resistance/vulnerability to oxidative stress (H2O2)
Response to doxorubicin (ROS-generating anti-tumor drug)
Activation of redox-responsive antioxidative and detoxification pathways
Upregulation of sirtuins
NRF2 signaling activity
Reactive oxygen species (ROS) defense

Limitations

In vitro cell culture model; findings may not translate to humans or in vivo tumors
Exposure duration not reported in the provided abstract
Sample size/replication details not reported in the provided abstract
Mechanistic claims are based on pathway/signaling observations as described in the abstract; full methods/results not provided here

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": null
  },
  "population": "SH-SY5Y human neuroblastoma cells",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation/hyperproliferation",
    "Cell survival advantage",
    "Resistance/vulnerability to oxidative stress (H2O2)",
    "Response to doxorubicin (ROS-generating anti-tumor drug)",
    "Activation of redox-responsive antioxidative and detoxification pathways",
    "Upregulation of sirtuins",
    "NRF2 signaling activity",
    "Reactive oxygen species (ROS) defense"
  ],
  "main_findings": "In SH-SY5Y human neuroblastoma cells, exposure to a 50 Hz, 1 mT extremely low frequency magnetic field induced hyperproliferation and a proliferative/survival advantage. The abstract reports activation of redox-responsive antioxidative and detoxification cytoprotective pathways (including increased NRF2 signaling and upregulation of sirtuins), reduced vulnerability to H2O2 and doxorubicin, and notes that effects on behavior and drug responses were also observed at 50 Hz as low as 100 µT.",
  "effect_direction": "harm",
  "limitations": [
    "In vitro cell culture model; findings may not translate to humans or in vivo tumors",
    "Exposure duration not reported in the provided abstract",
    "Sample size/replication details not reported in the provided abstract",
    "Mechanistic claims are based on pathway/signaling observations as described in the abstract; full methods/results not provided here"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency magnetic field",
    "ELF-MF",
    "power frequency",
    "50 Hz",
    "1 mT",
    "100 µT",
    "neuroblastoma",
    "SH-SY5Y",
    "malignant phenotype",
    "proliferation",
    "survival",
    "redox",
    "ROS",
    "NRF2",
    "sirtuins",
    "doxorubicin",
    "H2O2"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.35,
      "reason": "Study concerns power-frequency (50 Hz) magnetic field exposure, relevant to ELF/power-line and occupational contexts, though the model is in vitro."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1038/s41598-017-11869-8
PubMed: Not supplied

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