Effects of low intensity static electromagnetic radiofrequency fields on leiomyosarcoma and smooth muscle cell lines.

Hellenic journal of nuclear medicine · 2006 · Mixed · Low evidence

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MixedLow evidencePaper 5097

Study snapshot

PublicationHellenic journal of nuclear medicine · 2006

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

In this study we investigated the effects of low intensity static radiofrequency electromagnetic field (EMF) causing no thermal effects, on leiomyosarcoma cells (LSC), isolated from tumors of fifteen Wistar rats induced via a 3,4-benzopyrene injection. Electromagnetic resonance frequencies measurements and exposure of cells to static EMF were performed by a device called multi channel dynamic exciter 100 V1 (MCDE). The LSC were exposed to electromagnetic resonance radiofrequencies (ERF) between 10 kHz to 120 kHz, for 45 min. During a 24h period, after the exposure of the LSC to ERF, there was no inhibition of cells proliferation. In contrast, at the end of a 48 h incubation period, LSC proliferation dramatically decreased by more than 98% (P<0.001). At that time, the survived LSC were only 2% of the total cell population exposed to ERF, and under the same culture conditions showed significant decrease of proliferation. These cells were exposed once again to ERF for 45 min (totally 4 sessions of exposure, of 45 min duration each) and tested using a flow cytometer. Experiments as above were repeated five times. It was found that 45% of these double exposed to ERF, LSC (EMF cells) were apoptotic and only a small percentage 2%, underwent mitosis. In order to determinate their metastatic potential, these EMF cells were also counted and tested by an aggregometer for their ability to aggregate platelets and found to maintain this ability., since they showed no difference in platelet aggregation ability compared to the LSC not exposed to ERF (control cells). In conclusion, exposure of LSC to specific ERF, decreases their proliferation rate and induces cell apoptosis. Also, the LSC that survived after exposed to ERF, had a lower proliferation rate compared to the LSC controls (P<0.05) but did not loose their potential for metastases (platelet aggregation ability). The non-malignant SMC were not affected by the EMF exposure (P<0.4). The specific ERF generated from the MCDE electronic device, used in this study, is safe for humans and animals, according to the international safety standards.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationLeiomyosarcoma cells isolated from tumors of 15 Wistar rats; non-malignant smooth muscle cell lines (SMC)

Sample sizeNot supplied

Exposureunknown · 45 min per session; up to 4 sessions

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Leiomyosarcoma cells exposed to electromagnetic resonance radiofrequencies between 10 kHz and 120 kHz for 45 min showed no proliferation inhibition at 24 h, but at 48 h proliferation decreased by >98% (P<0.001). After repeat exposure (total 4 sessions), 45% of double-exposed cells were apoptotic and ~2% underwent mitosis; platelet aggregation ability did not differ versus controls. Non-malignant SMC were not affected (P<0.4).

Outcomes measured

Cell proliferation (24 h and 48 h post-exposure)
Apoptosis (flow cytometry)
Mitosis (flow cytometry)
Platelet aggregation ability (as metastatic potential proxy)
Effects on non-malignant smooth muscle cells (SMC)

Limitations

In vitro study; findings may not translate to in vivo or human exposures
Exposure described as 'static radiofrequency' and 'electromagnetic resonance radiofrequencies' without standard dosimetry metrics (e.g., SAR)
Sample size for cell experiments not reported in abstract (only that experiments were repeated five times)
Metastatic potential assessed indirectly via platelet aggregation assay

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "unknown",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "45 min per session; up to 4 sessions"
  },
  "population": "Leiomyosarcoma cells isolated from tumors of 15 Wistar rats; non-malignant smooth muscle cell lines (SMC)",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation (24 h and 48 h post-exposure)",
    "Apoptosis (flow cytometry)",
    "Mitosis (flow cytometry)",
    "Platelet aggregation ability (as metastatic potential proxy)",
    "Effects on non-malignant smooth muscle cells (SMC)"
  ],
  "main_findings": "Leiomyosarcoma cells exposed to electromagnetic resonance radiofrequencies between 10 kHz and 120 kHz for 45 min showed no proliferation inhibition at 24 h, but at 48 h proliferation decreased by >98% (P<0.001). After repeat exposure (total 4 sessions), 45% of double-exposed cells were apoptotic and ~2% underwent mitosis; platelet aggregation ability did not differ versus controls. Non-malignant SMC were not affected (P<0.4).",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study; findings may not translate to in vivo or human exposures",
    "Exposure described as 'static radiofrequency' and 'electromagnetic resonance radiofrequencies' without standard dosimetry metrics (e.g., SAR)",
    "Sample size for cell experiments not reported in abstract (only that experiments were repeated five times)",
    "Metastatic potential assessed indirectly via platelet aggregation assay"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "leiomyosarcoma",
    "smooth muscle cells",
    "in vitro",
    "electromagnetic field",
    "electromagnetic resonance frequencies",
    "10 kHz",
    "120 kHz",
    "apoptosis",
    "cell proliferation",
    "platelet aggregation"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 17160157

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